In Vitro Biology of Corneal Epithelium and Endothelium

Overview

Journal Trans Am Ophthalmol Soc

Specialty Ophthalmology

Date 1976 Jan 1

PMID 1246815

Citations 2

Authors

M YANOFF

Affiliations

Soon will be listed here.

Abstract

Four main areas are explored: (1) the proper culture medium for corneal tissue; (2) the effect of serum on in vitro tissue growth; (3) the in vitro interrelationships between corneal epithelium and endothelium; and (4) the biology of cultures of whole corneas (organ cultures). Modified Eagle's minimal essential medium (MEM) proved to be an excellent culture fluid. Corneal tissue could be grown in MEM without serum or clot, thus providing a defined culture medium. The in vitro biology of outgrowths of multilayered corneal epithelium and monolayered corneal endothelium are discussed. Contact inhibition between epithelium and endothelium is demonstrated in whole corneal (organ) cultures.

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References

Brown S, WELLER C, Vidrich A . Effect of corticosteroids on corneal collagenase of rabbits. Am J Ophthalmol. 1970; 70(5):744-7. DOI: 10.1016/0002-9394(70)90494-0. View

Dohlman C . The function of the corneal epithelium in health and disease. The Jonas S. Friedenwald Memorial Lecture. Invest Ophthalmol. 1971; 10(6):383-407. View

Donoff R, McLennan J, Grillo H . Preparation and properties of collagenases from epithelium and mesenchyme of healing mammalian wounds. Biochim Biophys Acta. 1971; 227(3):639-53. DOI: 10.1016/0005-2744(71)90014-3. View

Pfister R, McCulley J, Friend J, Dohlman C . Collagenase activity of intact corneal epithelium in peripheral alkali burns. Arch Ophthalmol. 1971; 86(3):308-13. DOI: 10.1001/archopht.1971.01000010310014. View

Johnson R, Sheridan J . Junctions between cancer cells in culture: ultrastructure and permeability. Science. 1971; 174(4010):717-9. DOI: 10.1126/science.174.4010.717. View

Brown S, Hook C, Tragakis M . Presence, significance, and inhibition of lysosomal proteoglycanases. Invest Ophthalmol. 1972; 11(3):149-52. View

Francois J, Cambie E, Feher J . Collagenase inhibition with penicillamine. Case report. Ophthalmologica. 1973; 166(3):222-5. DOI: 10.1159/000306814. View

Harris Jr E, Krane S . Collagenases (third of three parts). N Engl J Med. 1974; 291(13):652-61. DOI: 10.1056/NEJM197409262911305. View

Ho P, Davis W, Elliott J, Cohen S . Kinetics of corneal epithelial regeneration and epidermal growth factor. Invest Ophthalmol. 1974; 13(10):804-9. View

10.

Flaxman B, Chopra D, Harper R . Autoradiographic analysis of hormone-independent development of the mouse vaginal epithelium in organ culture. In Vitro. 1974; 10:42-50. DOI: 10.1007/BF02615337. View

11.

Gilula N, Reeves O, Steinbach A . Metabolic coupling, ionic coupling and cell contacts. Nature. 1972; 235(5336):262-5. DOI: 10.1038/235262a0. View

12.

Arya D, MANNAGH J, IRVINE Jr A . Effect of lysosomes on cultured rabbit corneal endothelial cells. Invest Ophthalmol. 1972; 11(8):662-7. View

13.

Martz E, Steinberg M . Contact inhibition of what? An analytical review. J Cell Physiol. 1973; 81(1):25-37. DOI: 10.1002/jcp.1040810104. View

14.

Harris A . Behavior of cultured cells on substrata of variable adhesiveness. Exp Cell Res. 1973; 77(1):285-97. DOI: 10.1016/0014-4827(73)90579-x. View

15.

Leuenberger P, Gnadinger M, Cabernard E . [On the synthesis of collagen in corneal epithelium. I. Electron-microscopic study on regenerated epithelium of the rabbit cornea]. Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1973; 187(3):171-82. DOI: 10.1007/BF00531116. View

16.

Newsome D, Takasugi M, Kenyon K, Stark W, Opelz G . Human corneal cells in vitro: morphology and histocompatibility (HL-A) antigens of pure cell populations. Invest Ophthalmol. 1974; 13(1):23-32. View

17.

Dipasquale A, Bell Jr P . The upper cell surface: its inability to support active cell movement in culture. J Cell Biol. 1974; 62(1):198-214. PMC: 2109182. DOI: 10.1083/jcb.62.1.198. View

18.

Flaxman B, Nelson B . Ultrastructural studies of the early junctional zone formed by keratinocytes showing contact inhibition of movement in vitro. J Invest Dermatol. 1974; 63(4):326-30. DOI: 10.1111/1523-1747.ep12680297. View

19.

Savage Jr C, Cohen S . Proliferation of corneal epithelium induced by epidermal growth factor. Exp Eye Res. 1973; 15(3):361-6. DOI: 10.1016/0014-4835(73)90151-6. View

20.

Flaxman B, LASFARGUES E . Hormone-independent DNA synthesis by epithelial cells of adult human mammary gland in organ culture. Proc Soc Exp Biol Med. 1973; 143(2):371-4. DOI: 10.3181/00379727-143-37323. View