Sensitivity of the Ultrasonic Interferometry Method (Echo-Cell) to Changes of Red Cell Aggregation: Application to Diabetes

The sensitivity of the ultrasonic interferometry method (Echo-Cell) to changes in red blood cell (RBC) aggregation was investigated in comparison to the Regulest erythroaggregometry known as a reference method. In experiments where different concentrations of dextrans of 40 or 70 kD molecular weights were added to normal RBCs, the Echo-Cell was proved as sensitive as erythroaggregometry. A comparative study using RBC samples from normal and diabetic subjects showed that the Echo-Cell was much more sensitive when aggregation was measured in diluted than undiluted plasma. The sensitivity of Echo-Cell measurements in diluted plasma was similar to that of erythroaggregometry. Further analysis revealed that RBC aggregation was underestimated by Echo-Cell when measurements were made in undiluted plasma containing high fibrinogen levels, implying that in that case an elevated plasma viscosity might indirectly affect the sensitivity of the Echo-Cell. The low sensitivity of the Echo-Cell to detect an abnormal RBC aggregation when suspensions were prepared in undiluted plasma, is likely related to a relatively high shear stress exerted on RBC aggregates by the suspending medium. In conclusion, the sensitivity of the Echo-Cell to detect abnormal changes in RBC aggregation can be optimized by diluting the plasma.