Loss of a 29-kilodalton Outer Membrane Protein in Acinetobacter Baumannii is Associated with Imipenem Resistance

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2002 Nov 28

PMID 12454194

Citations 54

Authors

Adriana S Limansky

Maria Alejandra Mussi

Alejandro M Viale

Affiliations

Soon will be listed here.

Abstract

We analyzed the possible causes of imipenem (IPM) resistance in multidrug-resistant isolates of Acinetobacter baumannii. Comparison of the outer membrane protein (OMP) profiles of two genomically related strains (Ab288 [IPM sensitive] and Ab242 [IPM resistant]) indicated the conspicuous loss of a 29-kDa polypeptide in the Ab242 strain. No carbapenemase activity was detected in any of these strains. The treatment of Ab288 with sodium salicylate resulted in IPM resistance and the loss of the 29-kDa OMP. In addition, IPM-resistant clones of Ab288 which were selected by repetitive culturing in increasing concentrations of this antibiotic also showed the absence of this 29-kDa OMP.

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References

Takahashi A, Yomoda S, Kobayashi I, Okubo T, Tsunoda M, Iyobe S . Detection of carbapenemase-producing Acinetobacter baumannii in a hospital. J Clin Microbiol. 2000; 38(2):526-9. PMC: 86139. DOI: 10.1128/JCM.38.2.526-529.2000. View

Silva G, Leitao G, Peixe L . Emergence of carbapenem-hydrolyzing enzymes in Acinetobacter baumannii clinical isolates. J Clin Microbiol. 1999; 37(6):2109-10. PMC: 85053. DOI: 10.1128/JCM.37.6.2109-2110.1999. View

Riccio M, Franceschini N, Boschi L, Caravelli B, Cornaglia G, Fontana R . Characterization of the metallo-beta-lactamase determinant of Acinetobacter baumannii AC-54/97 reveals the existence of bla(IMP) allelic variants carried by gene cassettes of different phylogeny. Antimicrob Agents Chemother. 2000; 44(5):1229-35. PMC: 89849. DOI: 10.1128/AAC.44.5.1229-1235.2000. View

Bou G, Cervero G, Dominguez M, Quereda C . Characterization of a nosocomial outbreak caused by a multiresistant Acinetobacter baumannii strain with a carbapenem-hydrolyzing enzyme: high-level carbapenem resistance in A. baumannii is not due solely to the presence of beta-lactamases. J Clin Microbiol. 2000; 38(9):3299-305. PMC: 87377. DOI: 10.1128/JCM.38.9.3299-3305.2000. View

Limansky A, Viale A . Can composition and structural features of oligonucleotides contribute to their wide-scale applicability as random PCR primers in mapping bacterial genome diversity?. J Microbiol Methods. 2002; 50(3):291-7. DOI: 10.1016/s0167-7012(02)00040-4. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Matsudaira P . Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem. 1987; 262(21):10035-8. View

Bouvet P, Grimont P . Identification and biotyping of clinical isolates of Acinetobacter. Ann Inst Pasteur Microbiol. 1987; 138(5):569-78. DOI: 10.1016/0769-2609(87)90042-1. View

Matagne A, Joris B, Erpicum T, Granier B, Frere J . The diversity of the catalytic properties of class A beta-lactamases. Biochem J. 1990; 265(1):131-46. PMC: 1136623. DOI: 10.1042/bj2650131. View

10.

Gehrlein M, Leying H, Cullmann W, Wendt S, Opferkuch W . Imipenem resistance in Acinetobacter baumanii is due to altered penicillin-binding proteins. Chemotherapy. 1991; 37(6):405-12. DOI: 10.1159/000238887. View

11.

Cohen S, Levy S, Foulds J, Rosner J . Salicylate induction of antibiotic resistance in Escherichia coli: activation of the mar operon and a mar-independent pathway. J Bacteriol. 1993; 175(24):7856-62. PMC: 206962. DOI: 10.1128/jb.175.24.7856-7862.1993. View

12.

Olsen G, Woese C, Overbeek R . The winds of (evolutionary) change: breathing new life into microbiology. J Bacteriol. 1994; 176(1):1-6. PMC: 205007. DOI: 10.1128/jb.176.1.1-6.1994. View

13.

Sumita Y, Fukasawa M . Transient carbapenem resistance induced by salicylate in Pseudomonas aeruginosa associated with suppression of outer membrane protein D2 synthesis. Antimicrob Agents Chemother. 1993; 37(12):2743-6. PMC: 192800. DOI: 10.1128/AAC.37.12.2743. View

14.

Hamzehpour M, Pechere J, Plesiat P, Kohler T . OprK and OprM define two genetically distinct multidrug efflux systems in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1995; 39(11):2392-6. PMC: 162953. DOI: 10.1128/AAC.39.11.2392. View

15.

Clark R . Imipenem resistance among Acinetobacter baumannii: association with reduced expression of a 33-36 kDa outer membrane protein. J Antimicrob Chemother. 1996; 38(2):245-51. DOI: 10.1093/jac/38.2.245. View

16.

Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W . Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25(17):3389-402. PMC: 146917. DOI: 10.1093/nar/25.17.3389. View

17.

Costa S, Woodcock J, Gill M, Wise R, Barone A, Caiaffa H . Outer-membrane proteins pattern and detection of beta-lactamases in clinical isolates of imipenem-resistant Acinetobacter baumannii from Brazil. Int J Antimicrob Agents. 2000; 13(3):175-82. DOI: 10.1016/s0924-8579(99)00123-5. View