Molecular Analysis of Sarcoidosis Tissues for Mycobacterium Species DNA

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2002 Nov 28

PMID 12453366

Citations 46

Authors

Wonder Puryear Drake

Zhiheng Pei

David T Pride

Robert D Collins

Timothy L Cover

Martin J Blaser

Affiliations

Soon will be listed here.

Abstract

We performed polymerase chain reaction analysis, for Mycobacterium species 16S rRNA, rpoB, and IS6110 sequences, on 25 tissue specimens from patients with sarcoidosis and on 25 control tissue specimens consisting of mediastinal or cervical lymph nodes and lung biopsies. Mycobacterium species 16S rRNA sequences were amplified from 12 (48%) rpoB sequences and from 6 (24%) of the sarcoidosis specimens. In total, 16S rRNA or rpoB sequences were amplified from 15 sarcoidosis specimens (60%) but were not detected in any of the control tissues (p=0.00002, chi square). In three specimens, the sequences resembled Mycobacterium species other than M. tuberculosis. All specimens with sequences consistent with M. tuberculosis were negative for IS6110. We provide evidence that one of a variety of Mycobacterium species, especially organisms resembling M. tuberculosis, is found in most patients with sarcoidosis.

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