Shedding of TNFR1 in Regenerative Liver Can Be Induced with TNF Alpha and PMA

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2002 Nov 20

PMID 12439939

Citations 12

Authors

Min Xia

Shao-Bai Xue

Cun-Shuan Xu

Affiliations

Soon will be listed here.

Abstract

Aim: Liver regeneration is associated with apoptosis of hepatocytes, which is mediated via tumor necrosis factor receptor 1(TNFR1). The shedding of TNFR1 in liver regeneration and its mechanism to regulate this shedding were investigated.

Methods: The shedding of TNFR1 in liver regeneration and changes of TNF-alpha, PMA and plasma membrane purified from hepatocytes on this shedding process were measured with Western blot. Then, the relationship between TNFR1 shedding and apoptosis of hepatocytes induced by TNFalpha was studied by detecting apoptotic index.

Results: The shedding of TNFR1 began at 4 hours and terminated before 2 months after partial hepatectomy. In culture system, serum from rats at 36 h after partial hepatectomy could also promote this shedding process. With the stimulation of TNFalpha, PMA or purified plasma membrane from hepatocytes at 36 h after partial hepatectomy or from hepatocytes treated with TNFalpha for 2 h, membranous TNFR1 was also shed. With the stimulation of both TNFalpha and plasma membrane from hepatocytes affected with TNFalpha for 2 h or from hepatocytes at 36 h after partial hepatectomy, apoptotic index of hepatocytes decreased from 21 % to 7.52 % and 8.45 %, respectively. PMA could also reduce apoptotic index to 13.67 %. This descent occurred in hepatocytes cultured in serum from rats at 36 h after partial hepatectomy too, but not in serum from rats at 2 months after partial hepatectomy and sham-operated rats.

Conclusion: Shedding of TNFR1 may help reduce apoptosis of hepatocytes induced by TNFalpha. Membrane-anchored metalloprotases could play a role in shedding membranous TNFR1. At the same time, PKC may take part in regulation of this shedding process.

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Deng M, Loughran P, Zhang L, Scott M, Billiar T Sci Signal. 2015; 8(361):ra11.

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References

Aicher B, Lerch M, Muller T, Schilling J, Ullrich A . Cellular redistribution of protein tyrosine phosphatases LAR and PTPsigma by inducible proteolytic processing. J Cell Biol. 1997; 138(3):681-96. PMC: 2141638. DOI: 10.1083/jcb.138.3.681. View

Decker K . Biologically active products of stimulated liver macrophages (Kupffer cells). Eur J Biochem. 1990; 192(2):245-61. DOI: 10.1111/j.1432-1033.1990.tb19222.x. View

Ramchandran R, Sen I . Cleavage processing of angiotensin-converting enzyme by a membrane-associated metalloprotease. Biochemistry. 1995; 34(39):12645-52. DOI: 10.1021/bi00039a021. View

Higuchi M, Aggarwal B . Inhibition of ligand binding and antiproliferative effects of tumor necrosis factor and lymphotoxin by soluble forms of recombinant P60 and P80 receptors. Biochem Biophys Res Commun. 1992; 182(2):638-43. DOI: 10.1016/0006-291x(92)91780-t. View

Huang E, Nocka K, Buck J, Besmer P . Differential expression and processing of two cell associated forms of the kit-ligand: KL-1 and KL-2. Mol Biol Cell. 1992; 3(3):349-62. PMC: 275535. DOI: 10.1091/mbc.3.3.349. View

Kohno T, Brewer M, Baker S, Schwartz P, King M, Hale K . A second tumor necrosis factor receptor gene product can shed a naturally occurring tumor necrosis factor inhibitor. Proc Natl Acad Sci U S A. 1990; 87(21):8331-5. PMC: 54949. DOI: 10.1073/pnas.87.21.8331. View

Porteu F, Brockhaus M, Wallach D, Engelmann H, Nathan C . Human neutrophil elastase releases a ligand-binding fragment from the 75-kDa tumor necrosis factor (TNF) receptor. Comparison with the proteolytic activity responsible for shedding of TNF receptors from stimulated neutrophils. J Biol Chem. 1991; 266(28):18846-53. View

Lloyd C, Ascoli M . On the mechanisms involved in the regulation of the cell-surface receptors for human choriogonadotropin and mouse epidermal growth factor in cultured Leydig tumor cells. J Cell Biol. 1983; 96(2):521-6. PMC: 2112295. DOI: 10.1083/jcb.96.2.521. View

Zhang L, Aggarwal B . Role of sulfhydryl groups in induction of cell surface down-modulation and shedding of extracellular domain of human TNF receptors in human histiocytic lymphoma U937 cells. J Immunol. 1994; 153(8):3745-54. View

10.

Stone A, Kroeger M, Sang Q . Structure-function analysis of the ADAM family of disintegrin-like and metalloproteinase-containing proteins (review). J Protein Chem. 1999; 18(4):447-65. DOI: 10.1023/a:1020692710029. View

11.

Sisodia S . Beta-amyloid precursor protein cleavage by a membrane-bound protease. Proc Natl Acad Sci U S A. 1992; 89(13):6075-9. PMC: 49440. DOI: 10.1073/pnas.89.13.6075. View

12.

Heldin C, Wasteson A, Westermark B . Interaction of platelet-derived growth factor with its fibroblast receptor. Demonstration of ligand degradation and receptor modulation. J Biol Chem. 1982; 257(8):4216-21. View

13.

Old L . Tumor necrosis factor (TNF). Science. 1985; 230(4726):630-2. DOI: 10.1126/science.2413547. View

14.

Higuchi M, Aggarwal B . TNF induces internalization of the p60 receptor and shedding of the p80 receptor. J Immunol. 1994; 152(7):3550-8. View

15.

West D, James N, Cosulich S, Holden P, Brindle R, Rolfe M . Role for tumor necrosis factor alpha receptor 1 and interleukin-1 receptor in the suppression of mouse hepatocyte apoptosis by the peroxisome proliferator nafenopin. Hepatology. 1999; 30(6):1417-24. DOI: 10.1002/hep.510300612. View

16.

Diehl A, Yin M, Fleckenstein J, Yang S, Lin H, Brenner D . Tumor necrosis factor-alpha induces c-jun during the regenerative response to liver injury. Am J Physiol. 1994; 267(4 Pt 1):G552-61. DOI: 10.1152/ajpgi.1994.267.4.G552. View

17.

Aggarwal B, Eessalu T . Effect of phorbol esters on down-regulation and redistribution of cell surface receptors for tumor necrosis factor-alpha. J Biol Chem. 1987; 262(34):16450-5. View

18.

Akerman P, Cote P, Yang S, McClain C, Nelson S, Bagby G . Antibodies to tumor necrosis factor-alpha inhibit liver regeneration after partial hepatectomy. Am J Physiol. 1992; 263(4 Pt 1):G579-85. DOI: 10.1152/ajpgi.1992.263.4.G579. View

19.

Selkoe D . Amyloid beta-protein and the genetics of Alzheimer's disease. J Biol Chem. 1996; 271(31):18295-8. DOI: 10.1074/jbc.271.31.18295. View

20.

Bradham C, Plumpe J, Manns M, Brenner D, Trautwein C . Mechanisms of hepatic toxicity. I. TNF-induced liver injury. Am J Physiol. 1998; 275(3):G387-92. DOI: 10.1152/ajpgi.1998.275.3.G387. View