Subpopulations of Mouse Spleen Lymphocytes. III. Cellular Interactions in the Response to Concanavalin A

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1975 Jun 1

PMID 124306

Authors

L Mugraby

I Gery

D Sulitzeanu

Affiliations

Soon will be listed here.

Abstract

The profile of response to concanavalin A (con A) of purified mouse T cells was found to differ appreciably from that of non-fractionated spleen cells, in agreement with results previously published by other investigators. Experiments designed to elucidate the reasons underlying these differences have revealed that the response of the spleen cells to con A is determined by a complex interplay between several cell types. (a) B cells contribute to the overall incorporation of thymidine in the presence of con A-stimulated T cells. However, the B cells participate in the response only if the T cells are dividing. (b) A population of 'adherent cells' is present in the spleen, which enhances the stimulation of the spleen cells by low doses of con A but suppresses the response to high doses of mitogen. These adherent cells include most likely the conventional macrophages, but probably also a population of 'suppressor T cells'. (c) Such 'suppressor T' cells can be readily detected among the peritoneal exudate cells. Addition of the exudate cells to cultures of purified T cells enhances the response to low doses of con A. This effect can be further increased by treating the peritoneal cells with a cell T-specific antiserum and complement, i.e. by eliminating the T cells.

References

Alter B, Bach F . Lymphocyte reactivity in vitro. I. Cellular reconstitution of purified lymphocyte response. Cell Immunol. 1970; 1(2):207-18. DOI: 10.1016/0008-8749(70)90008-0. View

Oppenheim J, Leventhal B, Hersh E . The transformation of column-purified lymphocytes with nonspecific and specific antigenic stimuli. J Immunol. 1968; 101(2):262-7. View

GERSHON R, Gery I, Waksman B . Suppressive effects of in vivo immunization on PHA responses in vitro. J Immunol. 1974; 112(1):215-21. View

UNANUE E . The regulatory role of macrophages in antigenic stimulation. Adv Immunol. 1972; 15:95-165. DOI: 10.1016/s0065-2776(08)60684-7. View

Folch H, Waksman B . The splenic suppressor cell. I. Activity of thymus-dependent adherent cells: changes with age and stress. J Immunol. 1974; 113(1):127-39. View

Golub E . Brain-associated theta antigen: reactivity of rabbit anti-mouse brain with mouse lymphoid cells. Cell Immunol. 1971; 2(4):353-61. DOI: 10.1016/0008-8749(71)90070-0. View

Scott M . Biological effects of the adjuvant Corynebacterium parvum. II. Evidence for macrophage-T-cell interaction. Cell Immunol. 1972; 5(3):469-79. DOI: 10.1016/0008-8749(72)90073-1. View

Vischer T, JAQUET C . Effect of antibodies against immunoglobulins and the theta antigen on the specific and non-specific stimulation of mouse spleen cells in vitro. Immunology. 1972; 22(2):259-66. PMC: 1408186. View

Elfenbein G, Shevach E, Green I . Proliferation by bone marrow-derived lymphocytes in response to antigenic stimulation in vitro. J Immunol. 1972; 109(4):870-4. View

10.

Andersson J, Moller G, Sjoberg O . B lymphocytes can be stimulated by concanavalin A in the presence of humoral factors released by T cells. Eur J Immunol. 1972; 2(1):99-101. DOI: 10.1002/eji.1830020119. View

11.

Shortman K, BYRD W, Cerottini J, BRUNNER K . Characterisation and separation of mouse lymphocyte subpopulations responding to phytohemagglutinin and pokeweed mitogens. Cell Immunol. 1973; 6(1):25-40. DOI: 10.1016/0008-8749(73)90003-8. View

12.

Andersson J, Edelman G, Moller G, Sjoberg O . Activation of B lymphocytes by locally concentrated concanavalin A. Eur J Immunol. 1972; 2(3):233-5. DOI: 10.1002/eji.1830020307. View

13.

Folch H, Yoshinaga M, Waksman B . Regulation of lymphocyte responses in vitro. 3. Inhibition by adherent cells of the T-lymphocyte response to phytohemagglutinin. J Immunol. 1973; 110(3):835-9. View

14.

Osborne Jr D, Katz D . Antigen-induced deoxyribonucleic acid synthesis in mouse lymphocytes. II. Analysis of the cell populations required for and responding to antigen stimulation in vitro. J Immunol. 1973; 111(4):1176-82. View

15.

Andersson J, Moller G, Sjoberg O . Selective induction of DNA synthesis in T and B lymphocytes. Cell Immunol. 1972; 4(4):381-93. DOI: 10.1016/0008-8749(72)90040-8. View

16.

Yoshinaga M, Yoshinaga A, Waksman B . Regulation of lymphocyte responses in vitro. I. Regulatory effect of macrophages and thymus-dependent (T) cells on the response of thymus-independent (B) lymphocytes to endotoxin. J Exp Med. 1972; 136(4):956-61. PMC: 2139268. DOI: 10.1084/jem.136.4.956. View

17.

Andersson J, Melchers F . Induction of immunoglobulin M synthesis and secretion in bone-marrow-derived lymphocytes by locally concentrated concanavalin A. Proc Natl Acad Sci U S A. 1973; 70(2):416-20. PMC: 433272. DOI: 10.1073/pnas.70.2.416. View

18.

Coutinho A, Moller G, Anderson J, Bullock W . In vitro activation of mouse lymphocytes in serum-free medium: effect of T and B cell mitogens on proliferation and antibody synthesis. Eur J Immunol. 1973; 3(5):299-306. DOI: 10.1002/eji.1830030509. View

19.

Greaves M, Janossy G . Elicitation of selective T and B lymphocyte responses by cell surface binding ligands. Transplant Rev. 1972; 11:87-130. DOI: 10.1111/j.1600-065x.1972.tb00047.x. View

20.

GERSHON R . T cell control of antibody production. Contemp Top Immunobiol. 1974; 3:1-40. DOI: 10.1007/978-1-4684-3045-5_1. View