In Vitro Culture of Functional Human Thymic Epithelium

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1975 Feb 1

PMID 1240047

Citations 19

Authors

M PAPIERNIK

B Nabarra

J F Bach

Affiliations

Soon will be listed here.

Abstract

Cultures of pure epithelial cells have been obtained from human thymus. Incubation of spleen cells from adult thymectomized mice on these cultures rendered RFC sensitive to inhibition by anti-theta serum, whereas no effect was obtained with fibroblasts or spleen cell cultures. The fact that theta conversion was obtained when spleen cells were incubated in the thymic culture placed within a Millipore chamber indicates that theta conversion was due to the effect of a humoral factor.

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References

Komuro K, Boyse E . In-vitro demonstration of thymic hormone in the mouse by conversion of precursor cells into lymphocytes. Lancet. 1973; 1(7806):740-3. DOI: 10.1016/s0140-6736(73)92127-2. View

Trainin N, Small M . Studies on some physicochemical properties of a thymus humoral factor conferring immunocompetence on lymphoid cells. J Exp Med. 1970; 132(5):885-97. PMC: 2138872. DOI: 10.1084/jem.132.5.885. View

Dardenne M, PAPIERNIK M, Bach J, Stutman O . Studies on thymus products. 3. Epithelial origin of the serum thymic factor. Immunology. 1974; 27(2):299-304. PMC: 1445565. View

Goldstein A, Guha A, Zatz M, Hardy M, White A . Purification and biological activity of thymosin, a hormone of the thymus gland. Proc Natl Acad Sci U S A. 1972; 69(7):1800-3. PMC: 426805. DOI: 10.1073/pnas.69.7.1800. View

Komuro K, Boyse E . Induction of T lymphocytes from precursor cells in vitro by a product of the thymus. J Exp Med. 1973; 138(2):479-82. PMC: 2139400. DOI: 10.1084/jem.138.2.479. View

Wekerle H, Cohen I, Feldman M . Thymus reticulum cell cultures confer T cell properties on spleen cells from thymus-deprived animals. Eur J Immunol. 1973; 3(12):745-8. DOI: 10.1002/eji.1830031202. View

CLARK Jr S . Incorporation of sulfate by the mouse thymus: its relation to secretion by medullary epithelial cells and to thymic lymphopoiesis. J Exp Med. 1968; 128(5):927-57. PMC: 2138565. DOI: 10.1084/jem.128.5.927. View

Bach J, Gigli I, Dardenne M, Dormont J . Mechanism of complement action in rosette inhibition and lymphocytotoxicity of antilymphocyte serum. Immunology. 1972; 22(4):625-35. PMC: 1407844. View

Raff M . Surface antigenic markers for distinguishing T and B lymphocytes in mice. Transplant Rev. 1971; 6:52-80. DOI: 10.1111/j.1600-065x.1971.tb00459.x. View

10.

Bach J, Dardenne M, Goldstein A, Guha A, White A . Appearance of T-cell markers in bone marrow rosette-forming cells after incubation with thymosin, a thymic hormone. Proc Natl Acad Sci U S A. 1971; 68(11):2734-8. PMC: 389512. DOI: 10.1073/pnas.68.11.2734. View

11.

Bach J, Dardenne M . Antigen recognition by T lymphocytes. 3. Evidence for two populations of thymus-dependent rosette-forming cells in spleen and lymph nodes. Cell Immunol. 1973; 6(3):394-406. DOI: 10.1016/0008-8749(73)90039-7. View

12.

Bach J, Dardenne M . Studies on thymus products. II. Demonstration and characterization of a circulating thymic hormone. Immunology. 1973; 25(3):353-66. PMC: 1423068. View

13.

Mosier D, Pierce C . Functional maturation of thymic lymphocyte populations in vitro. J Exp Med. 1972; 136(6):1484-500. PMC: 2139328. DOI: 10.1084/jem.136.6.1484. View

14.

Bach J, Dardenne M . Thymus dependency of rosette-forming cells: evidence for a circulating thymic hormone. Transplant Proc. 1972; 4(3):345-50. View