Lactate-glucose Interrelations, Glucose Recycling and the Cori Cycle in Normal Fed Rats

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1975 Dec 19

PMID 1239739

Citations 6

Authors

A Freminet

C Poyart

Affiliations

Soon will be listed here.

Abstract

1. Turnover and oxidation rates of glucose and lactate were determined using a priming dose-continuous infusion of 14C-(u)-glucose and 14C-(U)-lactate in anesthetized and mechanically ventilated non-fasted rats. The rates of glucose-lactate interconversions were computed from the two-compartment model of Depocas and De Freitas (1970). The rate of total glucose recycling is known as the difference between the rate of true glucose turnover measured with 3H-(2)-glucose (RGT) and the rate of apparent glucose turnover measured with 14C-glucose (RG). This value was compared with the Cori cycle. 2. In normal conditions 17% of RG come from lactate, 43% are directed to lactate and 49% are oxidized. 3. 71% of the rate of lactate turnover come from glucose, 28% are directed to glucose and 51% are oxidized. 4. The rate of total glucose recycling (RGT-RG) is 3.7 mg - mn(-1) per kg0.75 and represents 60% of RG or 38% of RGT. 5. The Cori cycle is 0.8 - 1.2 mg - mn(-1) per kg0.75 and represents 8 - 20% of the rate of glucose turnover and 20 - 32% of total glucose recycling.

Citing Articles

The Exposure to Different Photoperiods Strongly Modulates the Glucose and Lipid Metabolisms of Normoweight Fischer 344 Rats.

Marine-Casado R, Domenech-Coca C, Bas J, Blade C, Arola L, Caimari A Front Physiol. 2018; 9:416.

PMID: 29725308 PMC: 5917113. DOI: 10.3389/fphys.2018.00416.

The association of early combined lactate and glucose levels with subsequent renal and liver dysfunction and hospital mortality in critically ill patients.

Freire Jorge P, Wieringa N, de Felice E, van der Horst I, Oude Lansink A, Nijsten M Crit Care. 2017; 21(1):218.

PMID: 28826408 PMC: 5563890. DOI: 10.1186/s13054-017-1785-z.

Metabolic acidosis as a complication of intravenous dextrose administration in a patient with insulinoma.

Ramos-Prol A, Del Olmo-Garcia M, Perez-Lazaro A, Caballero-Soto M, Argente-Pla M, Leon-de Zayas B Endocrine. 2010; 38(3):402-5.

PMID: 20972720 DOI: 10.1007/s12020-010-9400-y.

Metabolism of 3H- and 14C-labelled lactate in starved rats.

Okajima F, Chenoweth M, ROGNSTAD R, Dunn A, Katz J Biochem J. 1981; 194(2):525-40.

PMID: 7306002 PMC: 1162777. DOI: 10.1042/bj1940525.

The determination of lactate turnover in vivo with 3H- and 14C-labelled lactate. The significance of sites of tracer administration and sampling.

Katz J, Okajima F, Chenoweth M, Dunn A Biochem J. 1981; 194(2):513-24.

PMID: 7306001 PMC: 1162776. DOI: 10.1042/bj1940513.

References

KREISBERG R, Pennington L, BOSHELL B . Lactate turnover and gluconeogenesis in normal and obese humans. Effect of starvation. Diabetes. 1970; 19(1):53-63. DOI: 10.2337/diab.19.1.53. View

Katz J, Dunn A, Chenoweth M, Golden S . Determination of synthesis, recycling and body mass of glucose in rats and rabbits in vivo 3H-and 14C-labelled glucose. Biochem J. 1974; 142(1):171-83. PMC: 1168223. DOI: 10.1042/bj1420171. View

Freminet A, Bursaux E, Poyart C . An improved micromethod for the determination of L-lactate 14 C in blood. Biochem Med. 1972; 6(1):72-6. DOI: 10.1016/0006-2944(72)90062-2. View

Argenzio R, HINTZ H . Effect of diet on glucose entry and oxidation rates in ponies. J Nutr. 1972; 102(7):879-92. DOI: 10.1093/jn/102.7.879. View

SEARLE G, Gulli R, CAVALIERI R . Effect of Phenformin in nondiabetic humans. Estimation of glucose turnover rate and Cori cycle activity. Metabolism. 1969; 18(2):148-54. DOI: 10.1016/0026-0495(69)90109-7. View

Waterhouse C, Keilson J . Cori cycle activity in man. J Clin Invest. 1969; 48(12):2359-66. PMC: 297493. DOI: 10.1172/JCI106202. View

Annison E, LINDSAY D, White R . METABOLIC INTERRELATIONS OF GLUCOSE AND LACTATE IN SHEEP. Biochem J. 1963; 88:243-8. PMC: 1202104. DOI: 10.1042/bj0880243. View

Ballard F, Hanson R, Kronfeld D . Gluconeogenesis and lipogenesis in tissue from ruminant and nonruminant animals. Fed Proc. 1969; 28(1):218-31. View

Clark D, ROGNSTAD R, Katz J . Isotopic evidence for futile cycles in liver cells. Biochem Biophys Res Commun. 1973; 54(3):1141-8. DOI: 10.1016/0006-291x(73)90811-5. View

10.

KREISBERG R . Glucose-lactate inter-relations in man. N Engl J Med. 1972; 287(3):132-7. DOI: 10.1056/NEJM197207202870307. View

11.

Park H, King F, MacLeod M, PITTS R . Metabolism of lactate by the intact functioning kidney of the dog. Am J Physiol. 1973; 224(6):1463-7. DOI: 10.1152/ajplegacy.1973.224.6.1463. View

12.

Brech W, Glennon J, Gordon E . [Kinetic studies of glucose metabolism. I. Glucose pool, glucose turnover and Cori cycle in normal volunteers]. Klin Wochenschr. 1970; 48(9):521-9. DOI: 10.1007/BF01488566. View

13.

Wahren J, FELIG P, Hendler R, Ahlborg G . Glucose and amino acid metabolism during recovery after exercise. J Appl Physiol. 1973; 34(6):838-45. DOI: 10.1152/jappl.1973.34.6.838. View

14.

Paul P, BORTZ W . Turnover and oxidation of plasma glucose in lean and obese humans. Metabolism. 1969; 18(7):570-84. DOI: 10.1016/0026-0495(69)90091-2. View

15.

VON HOLT , Schmidt H, Feldmann H, HALLMANN I . [The metabolism of blood glucose]. Biochem Z. 1961; 334:524-33. View

16.

Wiener R, SPITZER J . Glucose metabolism following severe hemorrhage in the conscious dog. Am J Physiol. 1974; 227(1):63-6. DOI: 10.1152/ajplegacy.1974.227.1.63. View

17.

Friedman B, GOODMAN Jr E, Saunders H, Kostos V, WEINHOUSE S . Estimation of pyruvate recycling during gluconeogenesis in perfused rat liver. Metabolism. 1971; 20(1):2-12. DOI: 10.1016/0026-0495(71)90055-2. View

18.

DEPOCAS F, MINAIRE Y, CHATONNET J . Rates of formation and oxidation of lactic acid in dogs at rest and during moderate exercise. Can J Physiol Pharmacol. 1969; 47(7):603-10. DOI: 10.1139/y69-106. View

19.

SCHMIDT-NIELSEN K . Energy metabolism, body size, and problems of scaling. Fed Proc. 1970; 29(4):1524-32. View

20.

Cerasi E, Wahren J, Luft R, FELIG P, Hendler R . The regulation of splanchnic glucose production in subjects with low insulin response--a compensatory mechanism in prediabetes?. Eur J Clin Invest. 1973; 3(3):193-200. DOI: 10.1111/j.1365-2362.1973.tb00349.x. View