Hepatic Stimulator Substance Activity in Animal Model of Fulminant Hepatic Failure and Encephalopathy

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 2002 Oct 25

PMID 12395888

Citations 7

Authors

Alexandra P Margeli

Evangelos Manolis

Spyridon N Skaltsas

Kyriakos S Tsarpalis

Michael G Mykoniatis

Stamatios E Theocharis

Affiliations

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Abstract

Hepatic stimulator substance (HSS) is a known liver-specific but species-nonspecific growth factor. In the present study we examined the activity of the endogenously produced HSS in an established experimental model of fulminant hepatic failure (FHF) and encephalopathy, induced by repeated injections of thioacetamide (TAA). FHF was induced by three consecutive intraperitoneal injections of TAA (400 mg/kg body weight) in rats, at time intervals of 24 hr. The animals were killed at 0, 6, 12, or 18 hr following the last injection of TAA. The rate of tritiated thymidine incorporation into hepatic DNA, the enzymatic activity of liver thymidine kinase (EC 2.7.1.21), and the assessment of mitotic index in hepatocytes were used to estimate liver regeneration. HSS extract obtained from the livers of TAA-treated rats, sacrificed at the above-mentioned time points was tested for its activity. Increased HSS activity was noted in all TAA-treated animals, presenting a peak at 12 hr following the third TAA dose, suggesting active participation of this growth factor in hepatocyte replication in this animal model of FHF and encephalopathy. It may also be suggested that up-regulation of HSS activity could be used in future as a therapeutic approach in FHF.

Citing Articles

Involvement of hepatic stimulator substance in experimentally induced fibrosis and cirrhosis in the rat.

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Platelet activating factor (PAF) antagonism with ginkgolide B protects the liver against acute injury. importance of controlling the receptor of PAF.

Grypioti A, Kostopanagiotou G, Demopoulos C, Roussos A, Mykoniatis M Dig Dis Sci. 2007; 53(4):1054-62.

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Platelet-activating factor inactivator (rPAF-AH) enhances liver's recovery after paracetamol intoxication.

Grypioti A, Kostopanagiotou G, Mykoniatis M Dig Dis Sci. 2007; 52(10):2580-90.

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Recombinant platelet-activating factor-acetylhydrolase attenuates paracetamol-induced liver oxidative stress, injury, and regeneration.

Grypioti A, Mykoniatis M, Demopoulos C, Kostopanagiotou G Dig Dis Sci. 2006; 52(1):192-9.

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During thioacetamide-induced acute liver failure, the proliferative response of hepatocytes to thyroid hormone is maintained, indicating a potential therapeutic approach to toxin-induced liver disease.

Malik R, Saich R, Rahman T, Hodgson H Dig Dis Sci. 2006; 51(12):2235-41.

PMID: 17080250 DOI: 10.1007/s10620-006-9275-1.

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