Article: Lactate dehydrogenase in preimplantation rabbit embryos

Unfertilized eggs and early embryos up to the 2-day (16-cell) cleavage stage of development in the rabbit contain predominantly the most cathodal lactate dehydrogenase isoenzyme made up of A-type subunits. Following early cleavage there is a progressing increase in total LDH activity in the embryo as development proceeds through 4- and 6-day blastocyst stages. This is accompanied by an increase in the amount of B-type subunits and a concomitant shift in the lactate dehydrogenase isoenzyme electrophoretic pattern toward the anodal isoenzyme types.

Citing Articles

Lactate dehydrogenase in one-cell rabbit embryos extracted with Triton X-100.

Peacock T, Schultz G, BROWDER L Biochem Genet. 1976; 14(5-6):523-5.

PMID: 971295 DOI: 10.1007/BF00486132.

References

1.

Engel W, Petzoldt U . Early developmental changes of the lactate dehydrogenase isoenzyme pattern in mouse, rat, guinea-pig, Syrian hamster and rabbit. Humangenetik. 1973; 20(2):125-31. DOI: 10.1007/BF00284847. View

2.

Auerbach S, Brinster R . Lactate dehydrogenase isozymes in the early mouse embryo. Exp Cell Res. 1967; 46(1):89-92. DOI: 10.1016/0014-4827(67)90411-9. View

3.

Brinster R . Lactate dehydrogenase isozymes in the preimplantation rabbit embryo. Biochem Genet. 1973; 9(3):229-34. DOI: 10.1007/BF00485736. View

4.

Brinster R . Lactate dehydrogenase activity in the preimplantation rabbit embryo. Biochim Biophys Acta. 1967; 148(1):298-300. DOI: 10.1016/0304-4165(67)90306-6. View

5.

Schultz G, RUTH R . The lactate dehydrogenases of the chicken: estimation and repression during the development of lymphoid and other tissues. Can J Biochem. 1968; 46(6):555-62. DOI: 10.1139/o68-086. View

6.

Engel W, Kreutz R . Lactate dehydrogenase isoenzymes in the mammalian egg: investigations by micro disc electrophoresis in 15 species of the orders Rodentia, Lagomorpha, Carnivora, Artiodactyla and in man. Humangenetik. 1973; 19(3):253-60. DOI: 10.1007/BF00278398. View

7.

Brinster R . Lactate dehydrogenase activity in the preimplanted mouse embryo. Biochim Biophys Acta. 1965; 110(2):439-41. DOI: 10.1016/s0926-6593(65)80056-x. View

8.

Brinster R . Lactate dehydrogenase activity in the oocytes of mammals. J Reprod Fertil. 1968; 17(1):139-46. DOI: 10.1530/jrf.0.0170139. View

9.

Manes C . Nucleic acid synthesis in preimplantation rabbit embryos. I. Quantitative aspects, relationship to early morphogenesis and protein synthesis. J Exp Zool. 1969; 172(3):303-10. DOI: 10.1002/jez.1401720305. View

10.

HORECKER B, Kornberg A . The extinction coefficients of the reduced band of pyridine nucleotides. J Biol Chem. 1948; 175(1):385-90. View

11.

Rapola J, KOSKIMIES O . Embryonic enzyme patterns: characterization of the single lactate dehydrogenase isozyme in preimplanted mouse ova. Science. 1967; 157(3794):1311-2. DOI: 10.1126/science.157.3794.1311. View

Lactate Dehydrogenase in Preimplantation Rabbit Embryos