Variability of Cell Proliferation in the Proximal and Distal Colon of Normal Rats and Rats with Dimethylhydrazine Induced Carcinogenesis

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2002 Oct 16

PMID 12378628

Citations 8

Authors

Qing-Yong Ma

Kate E Williamson

Brian J Rowlands

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the patterns of cell proliferation in proximal and distal colons in normal rats and rats with 1,2-dimethylhydrazine (DMH) induced carcinogenesis using the thymidine analogue bromodeoxyuridine.

Methods: Colonic crypt cell proliferation was immunohistochemically detected using the anti-bromodeoxyuridine Bu20a monoclonal antibody.

Results: Marked regional differences were found in both groups. Total labelling index (LI) and proliferative zone size in both normal (8.65+/-0.34 vs 7.2+/-0.45, 27.74+/-1.07 vs 16.75+/-1.45) and DMH groups (13.13+/-0.46 vs 11.55+/-0.45, 39.60+/-1.32 vs 35.52+/-1.58) were significantly higher in distal than in proximal colon (P<0.05), although the number of cells per proximal crypt was greater (31.45+/-0.20 vs 34.45 +/-0.39, 42.68+/-0.53 vs 49.09+/-0.65, P<0.0001). Crypt length, total LI and proliferative zone size all increased in both proximal and distal regions of DMH rats compared to normal controls (P<0.0001). In DMH-treated rat colon a shift of labelled cells to higher crypt cell positions was demonstrated distally whilst a bi-directional shift was evident proximally (P<0.05).

Conclusion: Our results show that changes in cell proliferation patterns, as assessed by bromodeoxyuridine uptake, can act as a reliable intermediate marker of colonic cancer formation. Observed differences between proliferation patterns in distal and proximal colon may be associated with the higher incidence of tumors in the distal colon.

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References

Wong W, Wright N . Cell proliferation in gastrointestinal mucosa. J Clin Pathol. 1999; 52(5):321-33. PMC: 1023065. DOI: 10.1136/jcp.52.5.321. View

Stammberger P, Baczako K . Cytokeratin 19 expression in human gastrointestinal mucosa during human prenatal development and in gastrointestinal tumours: relation to cell proliferation. Cell Tissue Res. 1999; 298(2):377-81. DOI: 10.1007/s004419900085. View

Richards T . Early changes in the dynamics of crypt cell populations in mouse colon following administration of 1,2-dimethylhydrazine. Cancer Res. 1977; 37(6):1680-5. View

Xie B, Wang X . Effect of gastrin on protein kinase C and its subtype in human colon cancer cell line SW480. World J Gastroenterol. 2002; 6(2):304-306. PMC: 4723513. DOI: 10.3748/wjg.v6.i2.304. View

Sun K, Jin B, Feng Q, Zhu Y, Yang K, Liu X . Identification of CD226 ligand on colo205 cell surface. World J Gastroenterol. 2002; 8(1):108-13. PMC: 4656598. DOI: 10.3748/wjg.v8.i1.108. View

Ma Q, Hoper M, Anderson N, Rowlands B . Effect of supplemental L-arginine in a chemical-induced model of colorectal cancer. World J Surg. 1996; 20(8):1087-91. DOI: 10.1007/s002689900165. View

Colussi C, Fiumicino S, Giuliani A, Rosini S, Musiani P, Macri C . 1,2-Dimethylhydrazine-induced colon carcinoma and lymphoma in msh2(-/-) mice. J Natl Cancer Inst. 2001; 93(20):1534-40. DOI: 10.1093/jnci/93.20.1534. View

Aly A, Shulkes A, Baldwin G . Short term infusion of glycine-extended gastrin(17) stimulates both proliferation and formation of aberrant crypt foci in rat colonic mucosa. Int J Cancer. 2001; 94(3):307-13. DOI: 10.1002/ijc.1483. View

Chapkin R, Lupton J . Colonic cell proliferation and apoptosis in rodent species. Modulation by diet. Adv Exp Med Biol. 2000; 470:105-18. DOI: 10.1007/978-1-4615-4149-3_12. View

10.

Chang W, Leblond C . Renewal of the epithelium in the descending colon of the mouse. I. Presence of three cell populations: vacuolated-columnar, mucous and argentaffin. Am J Anat. 1971; 131(1):73-99. DOI: 10.1002/aja.1001310105. View

11.

Cascinu S, Ligi M, Del Ferro E, Foglietti G, Cioccolini P, Staccioli M . Effects of calcium and vitamin supplementation on colon cell proliferation in colorectal cancer. Cancer Invest. 2000; 18(5):411-6. DOI: 10.3109/07357900009032811. View

12.

Wilson R, Smith A, Bird C . Immunohistochemical detection of abnormal cell proliferation in colonic mucosa of subjects with polyps. J Clin Pathol. 1990; 43(9):744-7. PMC: 502753. DOI: 10.1136/jcp.43.9.744. View

13.

Mills S, Mathers J, Chapman P, Burn J, Gunn A . Colonic crypt cell proliferation state assessed by whole crypt microdissection in sporadic neoplasia and familial adenomatous polyposis. Gut. 2000; 48(1):41-6. PMC: 1728170. DOI: 10.1136/gut.48.1.41. View

14.

Ochsenkuhn T, Bayerdorffer E, Meining A, Schinkel M, Thiede C, Nussler V . Colonic mucosal proliferation is related to serum deoxycholic acid levels. Cancer. 1999; 85(8):1664-9. View

15.

Qin Y, Willems G . Comparison of the classical autoradiographic and the immunohistochemical methods with BrdU for measuring proliferation parameters in colon cancer. Anticancer Res. 1993; 13(3):731-5. View

16.

Jenab M, Thompson L . Phytic acid in wheat bran affects colon morphology, cell differentiation and apoptosis. Carcinogenesis. 2000; 21(8):1547-52. View

17.

Liu Z, Uesaka T, Watanabe H, Kato N . High fat diet enhances colonic cell proliferation and carcinogenesis in rats by elevating serum leptin. Int J Oncol. 2001; 19(5):1009-14. DOI: 10.3892/ijo.19.5.1009. View

18.

Davidson L, Brown R, Chang W, Morris J, Wang N, Carroll R . Morphodensitometric analysis of protein kinase C beta(II) expression in rat colon: modulation by diet and relation to in situ cell proliferation and apoptosis. Carcinogenesis. 2000; 21(8):1513-9. View

19.

Xiao B, Jing B, Zhang Y, Zhou D, Zhang W . Tumor growth inhibition effect of hIL-6 on colon cancer cells transfected with the target gene by retroviral vector. World J Gastroenterol. 2002; 6(1):89-92. PMC: 4723605. DOI: 10.3748/wjg.v6.i1.89. View

20.

Barnes C, Hardman W, Cameron I . Presence of well-differentiated distal, but not poorly differentiated proximal, rat colon carcinomas is correlated with increased cell proliferation in and lengthening of colon crypts. Int J Cancer. 1999; 80(1):68-71. DOI: 10.1002/(sici)1097-0215(19990105)80:1<68::aid-ijc14>3.0.co;2-0. View