The Gene YjfQ Encodes the Repressor of the YjfR-X Regulon (ula), Which is Involved in L-ascorbate Metabolism in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2002 Oct 11

PMID 12374842

Citations 7

Authors

Evangelina Campos

Juan Aguilar

Laura Baldoma

Josefa Badia

Affiliations

Soon will be listed here.

Abstract

Mutations in yjfQ allowed us to identify this gene as the regulator of the operon yjfS-X (ula operon), reported to be involved in L-ascorbate metabolism. Inactivation of this gene renders constitutive the expression of the ula operon, indicating that YjfQ acts as a repressor. We also demonstrate that this repressor regulates the nearby yjfR gene, which in this way constitutes a regulon with the ula operon.

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Structure of L-xylulose-5-Phosphate 3-epimerase (UlaE) from the anaerobic L-ascorbate utilization pathway of Escherichia coli: identification of a novel phosphate binding motif within a TIM barrel fold.

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The yiaKLX1X2PQRS and ulaABCDEFG gene systems are required for the aerobic utilization of L-ascorbate in Klebsiella pneumoniae strain 13882 with L-ascorbate-6-phosphate as the inducer.

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Overproduction, crystallization and preliminary X-ray analysis of the putative L-ascorbate-6-phosphate lactonase UlaG from Escherichia coli.

Garces F, Fernandez F, Perez-Luque R, Aguilar J, Baldoma L, Coll M Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007; 64(Pt 1):36-8.

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