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Inhibition of in Vitro Pollen Tube Growth by Isolated S-Glycoproteins of Nicotiana Alata

Overview
Journal Plant Cell
Publisher Oxford University Press
Specialties Biology
Cell Biology
Date 1989 May 1
PMID 12359898
Citations 33
Authors
W Jahnen
W M Lush
A E Clarke
Affiliations
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Abstract

Pollen from three S-genotypes of Nicotiana alata was grown in vitro in the presence of S-glycoproteins isolated from styles of the same three genotypes. Pollen germination was not affected by the presence of the S-glycoproteins, but pollen tube growth of all genotypes was inhibited. S2 pollen was preferentially inhibited by the S2-glycoprotein and S3 pollen by the S3-glycoprotein. The S6-glycoprotein preferentially inhibited growth of both S2 and S6 pollen over S3 pollen. Heat treatment dramatically increased the inhibitory activity of the S-glycoproteins as inhibitors both of pollen germination and tube growth; after heat treatment, S-allele specificity of pollen tube inhibition was not detected.

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