Localization of Hydrogen Peroxide Production in Pisum Sativum L. Using Epi-Polarization Microscopy to Follow Cerium Perhydroxide Deposition

Overview

Journal Plant Physiol

Specialty Physiology

Date 1995 Feb 1

PMID 12228377

Citations 2

Authors

L Liu

KEL. Eriksson

JFD. Dean

Affiliations

Soon will be listed here.

Abstract

Cerium is becoming an increasingly popular reagent for histochemical localization of oxidases and phosphatases because it combines directly with reaction products to form fine precipitates of electron-dense materials that can be easily detected using transmission electron microscopy or laser confocal scanning microscopy. We used epi-polarization microscopy to detect cerium perhydroxide deposits formed when H2O2 was produced by diamine oxidase in pea (Pisum sativum L.) epicotyls exposed to exogenous putrescine. Diamine oxidase activity was abundant in cortical cell walls but showed little, if any, association with vascular tissues. Maps of cerium deposition generated using scanning electron microscopy/x-ray microanalysis verified these observations. This study demonstrates the use of epi-polarization microscopy to follow cerium deposition, and the ready accessibility of this microscopy technique should facilitate more widespread use of cerium for plant histochemistry and cytochemistry.

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References

Okuda T, Matsuda Y, Yamanaka A, Sagisaka S . Abrupt increase in the level of hydrogen peroxide in leaves of winter wheat is caused by cold treatment. Plant Physiol. 1991; 97(3):1265-7. PMC: 1081153. DOI: 10.1104/pp.97.3.1265. View

Van Noorden C, Frederiks W . Cerium methods for light and electron microscopical histochemistry. J Microsc. 1993; 171(Pt 1):3-16. DOI: 10.1111/j.1365-2818.1993.tb03354.x. View

Robinson J, Batten B . Localization of cerium-based reaction products by scanning laser reflectance confocal microscopy. J Histochem Cytochem. 1990; 38(3):315-8. DOI: 10.1177/38.3.2406336. View

Brightman A, Barr R, Crane F, Morre D . Auxin-Stimulated NADH Oxidase Purified from Plasma Membrane of Soybean. Plant Physiol. 1988; 86(4):1264-9. PMC: 1054662. DOI: 10.1104/pp.86.4.1264. View

Bradley D, Kjellbom P, Lamb C . Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response. Cell. 1992; 70(1):21-30. DOI: 10.1016/0092-8674(92)90530-p. View

Halbhuber K, Hulstaert C, FEUERSTEIN H, Zimmermann N . Cerium as capturing agent in phosphatase and oxidase histochemistry. Theoretical background and applications. Prog Histochem Cytochem. 1994; 28(1):1-120. DOI: 10.1016/s0079-6336(11)80041-0. View

Schopfer P . Histochemical Demonstration and Localization of H2O2 in Organs of Higher Plants by Tissue Printing on Nitrocellulose Paper. Plant Physiol. 1994; 104(4):1269-1275. PMC: 159290. DOI: 10.1104/pp.104.4.1269. View

Chen Z, Silva H, Klessig D . Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid. Science. 1993; 262(5141):1883-6. DOI: 10.1126/science.8266079. View

Briggs R, Drath D, Karnovsky M, Karnovsky M . Localization of NADH oxidase on the surface of human polymorphonuclear leukocytes by a new cytochemical method. J Cell Biol. 1975; 67(3):566-86. PMC: 2111669. DOI: 10.1083/jcb.67.3.566. View

10.

Mehdy M . Active Oxygen Species in Plant Defense against Pathogens. Plant Physiol. 1994; 105(2):467-472. PMC: 159383. DOI: 10.1104/pp.105.2.467. View

11.

Church D, Galston A . 4-Coumarate:coenzyme A ligase and isoperoxidase expression in Zinnia mesophyll cells induced to differentiate into tracheary elements. Plant Physiol. 1988; 88:679-84. PMC: 1055644. DOI: 10.1104/pp.88.3.679. View

12.

LANE B . Oxalate, germin, and the extracellular matrix of higher plants. FASEB J. 1994; 8(3):294-301. DOI: 10.1096/fasebj.8.3.8143935. View

13.

Rapaport D, Herman K, LaVail M . Epi-polarization and incident light microscopy readily resolve an autoradiographic or heavy metal label from an obscuring background or second label. J Neurosci Methods. 1992; 41(3):231-8. DOI: 10.1016/0165-0270(92)90088-u. View

14.

Scandalios J . Oxygen Stress and Superoxide Dismutases. Plant Physiol. 1993; 101(1):7-12. PMC: 158641. DOI: 10.1104/pp.101.1.7. View

15.

Kaur-Sawhney R, Flores H, Galston A . Polyamine oxidase in oat leaves: a cell wall-localized enzyme. Plant Physiol. 1981; 68(2):494-8. PMC: 427517. DOI: 10.1104/pp.68.2.494. View

16.

Cooper J, Varner J . Cross-linking of soluble extensin in isolated cell walls. Plant Physiol. 1984; 76(2):414-7. PMC: 1064302. DOI: 10.1104/pp.76.2.414. View

17.

Okada T, Robinson J, Karnovsky M . Cytochemical localization of acid phosphatase in striated muscle. Histochemistry. 1986; 85(3):177-83. DOI: 10.1007/BF00494801. View

18.

De Waele M, Renmans W, Segers E, Jochmans K, Van Camp B . Sensitive detection of immunogold-silver staining with darkfield and epi-polarization microscopy. J Histochem Cytochem. 1988; 36(6):679-83. DOI: 10.1177/36.6.3259250. View

19.

Smith T . Polyamine oxidase in higher plants. Biochem Biophys Res Commun. 1970; 41(6):1452-6. DOI: 10.1016/0006-291x(70)90549-8. View