Ultraviolet B-Sensitive Rice Cultivar Deficient in Cyclobutyl Pyrimidine Dimer Repair

Overview

Journal Plant Physiol

Specialty Physiology

Date 1997 Jan 1

PMID 12223592

Citations 16

Authors

J Hidema

T Kumagai

J C SUTHERLAND

B M Sutherland

Affiliations

Soon will be listed here.

Abstract

Repair of cyclobutyl pyrimidine dimers (CPDs) in DNA is essential in most organisms to prevent biological damage by ultraviolet (UV) light. In higher plants tested thus far, UV-sensitive strains had higher initial damage levels or deficient repair of nondimer DNA lesions but normal CPD repair. This suggested that CPDs might not be important for biological lesions. The photosynthetic apparatus has also been proposed as a critical target. We have analyzed CPD induction and repair in the UV-sensitive rice (Oryza sativa L.) cultivar Norin 1 and its close relative UV-resistant Sasanishiki using alkaline agarose gel electrophoresis. Norin 1 is deficient in cyclobutyl pyrimidine dimer photoreactivation and excision; thus, UV sensitivity correlates with deficient dimer repair.

Citing Articles

High ultraviolet-B sensitivity due to lower CPD photolyase activity is needed for biotic stress response to the rice blast fungus, Magnaporthe oryzae.

Mmbando G, Ando S, Takahashi H, Hidema J Photochem Photobiol Sci. 2023; 22(6):1309-1321.

PMID: 36729358 DOI: 10.1007/s43630-023-00379-4.

Transgenic rice with higher CPD photolyase activity alleviates UVB-caused growth inhibition.

Mmbando G, Teranishi M, Hidema J GM Crops Food. 2021; 12(1):435-448.

PMID: 34935587 PMC: 8820246. DOI: 10.1080/21645698.2021.1977068.

Structural Aspects of DNA Repair and Recombination in Crop Improvement.

Verma P, Tandon R, Yadav G, Gaur V Front Genet. 2020; 11:574549.

PMID: 33024442 PMC: 7516265. DOI: 10.3389/fgene.2020.574549.

Very high sensitivity of African rice to artificial ultraviolet-B radiation caused by genotype and quantity of cyclobutane pyrimidine dimer photolyase.

Mmbando G, Teranishi M, Hidema J Sci Rep. 2020; 10(1):3158.

PMID: 32081870 PMC: 7035317. DOI: 10.1038/s41598-020-59720-x.

Ultraviolet-B-induced DNA damage and ultraviolet-B tolerance mechanisms in species with different functional groups coexisting in subalpine moorlands.

Wang Q, Kamiyama C, Hidema J, Hikosaka K Oecologia. 2016; 181(4):1069-82.

PMID: 27139425 DOI: 10.1007/s00442-016-3644-z.

References

Quaite F, Sutherland B, SUTHERLAND J . Quantitation of pyrimidine dimers in DNA from UVB-irradiated alfalfa (Medicago sativa L.) seedlings. Appl Theor Electrophor. 1992; 2(6):171-5. View

Bennett P, Sutherland B . Quantitative detection of single-copy genes in nanogram samples of human genomic DNA. Biotechniques. 1993; 15(3):520-5. View

Sutherland B, Bennett P . Human white blood cells contain cyclobutyl pyrimidine dimer photolyase. Proc Natl Acad Sci U S A. 1995; 92(21):9732-6. PMC: 40876. DOI: 10.1073/pnas.92.21.9732. View

Li J, Ou-Lee T, Raba R, Amundson R, Last R . Arabidopsis Flavonoid Mutants Are Hypersensitive to UV-B Irradiation. Plant Cell. 1993; 5(2):171-179. PMC: 160260. DOI: 10.1105/tpc.5.2.171. View

Sutherland B, Bennett P, Conlon K, Epling G, SUTHERLAND J . Quantitation of supercoiled DNA cleavage in nonradioactive DNA: application to ionizing radiation and synthetic endonuclease cleavage. Anal Biochem. 1992; 201(1):80-6. DOI: 10.1016/0003-2697(92)90176-8. View

Lao K, Glazer A . Ultraviolet-B photodestruction of a light-harvesting complex. Proc Natl Acad Sci U S A. 1996; 93(11):5258-63. PMC: 39232. DOI: 10.1073/pnas.93.11.5258. View

SUTHERLAND J, Lin B, Monteleone D, Mugavero J, Sutherland B, Trunk J . Electronic imaging system for direct and rapid quantitation of fluorescence from electrophoretic gels: application to ethidium bromide-stained DNA. Anal Biochem. 1987; 163(2):446-57. DOI: 10.1016/0003-2697(87)90247-8. View

Trosko J, Mansour V . Response of tobacco and Haplopappus cells to ultraviolet irradiation after posttreatment with photoreactivating light. Radiat Res. 1968; 36(2):333-43. View

FREEMAN S, Blackett A, Monteleone D, Setlow R, Sutherland B, SUTHERLAND J . Quantitation of radiation-, chemical-, or enzyme-induced single strand breaks in nonradioactive DNA by alkaline gel electrophoresis: application to pyrimidine dimers. Anal Biochem. 1986; 158(1):119-29. DOI: 10.1016/0003-2697(86)90599-3. View

10.

Britt A . DNA DAMAGE AND REPAIR IN PLANTS. Annu Rev Plant Physiol Plant Mol Biol. 1996; 47:75-100. DOI: 10.1146/annurev.arplant.47.1.75. View

11.

Brash D, Seetharam S, Kraemer K, Seidman M, Bredberg A . Photoproduct frequency is not the major determinant of UV base substitution hot spots or cold spots in human cells. Proc Natl Acad Sci U S A. 1987; 84(11):3782-6. PMC: 304960. DOI: 10.1073/pnas.84.11.3782. View

12.

Ziegler A, Leffell D, Kunala S, Sharma H, Gailani M, Simon J . Mutation hotspots due to sunlight in the p53 gene of nonmelanoma skin cancers. Proc Natl Acad Sci U S A. 1993; 90(9):4216-20. PMC: 46477. DOI: 10.1073/pnas.90.9.4216. View

13.

Quaite F, SUTHERLAND J, Sutherland B . Isolation of high-molecular-weight plant DNA for DNA damage quantitation: relative effects of solar 297 nm UVB and 365 nm radiation. Plant Mol Biol. 1994; 24(3):475-83. DOI: 10.1007/BF00024115. View

14.

Brash D, Rudolph J, Simon J, Lin A, McKenna G, Baden H . A role for sunlight in skin cancer: UV-induced p53 mutations in squamous cell carcinoma. Proc Natl Acad Sci U S A. 1991; 88(22):10124-8. PMC: 52880. DOI: 10.1073/pnas.88.22.10124. View

15.

Britt A, Chen J, Wykoff D, Mitchell D . A UV-sensitive mutant of Arabidopsis defective in the repair of pyrimidine-pyrimidinone(6-4) dimers. Science. 1993; 261(5128):1571-4. DOI: 10.1126/science.8372351. View

16.

Trosko J, Mansour V . Photoreactivation of ultraviolet light-induced pyrimidine dimers in Ginkgo cells grown in vitro. Mutat Res. 1969; 7(1):120-1. DOI: 10.1016/0027-5107(69)90056-6. View

17.

Pang Q, Hays J . UV-B-Inducible and Temperature-Sensitive Photoreactivation of Cyclobutane Pyrimidine Dimers in Arabidopsis thaliana. Plant Physiol. 1991; 95(2):536-43. PMC: 1077565. DOI: 10.1104/pp.95.2.536. View

18.

SUTHERLAND J, Monteleone D, Mugavero J, Trunk J . Unidirectional pulsed-field electrophoresis of single- and double-stranded DNA in agarose gels: analytical expressions relating mobility and molecular length and their application in the measurement of strand breaks. Anal Biochem. 1987; 162(2):511-20. DOI: 10.1016/0003-2697(87)90427-1. View

19.

Ziegler A, Jonason A, Leffell D, Simon J, Sharma H, Kimmelman J . Sunburn and p53 in the onset of skin cancer. Nature. 1994; 372(6508):773-6. DOI: 10.1038/372773a0. View