The Escherichia Coli MetD Locus Encodes an ABC Transporter Which Includes Abc (MetN), YaeE (MetI), and YaeC (MetQ)

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2002 Sep 10

PMID 12218041

Citations 55

Authors

Christophe Merlin

Gregory Gardiner

Sylvain Durand

Millicent Masters

Affiliations

Soon will be listed here.

Abstract

We report that the genes abc, yaeC, and yaeE comprise metD, an Escherichia coli locus encoding a DL-methionine uptake system. MetD is an ABC transporter with Abc the ATPase, YaeE the permease, and YaeC the likely substrate binding protein. Expression of these genes is regulated by L-methionine and MetJ, a common repressor of the methionine regulon. We propose to rename abc, yaeE, and yaeC as metN, metI, and metQ, respectively.

Citing Articles

Two stages of substrate discrimination dictate selectivity in the MetNI-Q ABC transporter system.

Yang J, Chen H, Guardado J, Gardner M, Foronda M bioRxiv. 2025; .

PMID: 39896590 PMC: 11785062. DOI: 10.1101/2025.01.20.633972.

Small RNA CjNC110 regulates the activated methyl cycle to enable optimal chicken colonization by .

Ruddell B, Hassall A, Sahin O, Plummer P, Zhang Q, Kreuder A mSphere. 2025; 10(1):e0083224.

PMID: 39772717 PMC: 11774046. DOI: 10.1128/msphere.00832-24.

Flipping the switch: dynamic modulation of membrane transporter activity in bacteria.

Elston R, Mulligan C, Thomas G Microbiology (Reading). 2023; 169(11).

PMID: 37948297 PMC: 10710835. DOI: 10.1099/mic.0.001412.

Disruption of the metC Gene Affects Methionine Biosynthesis in Pectobacterium carotovorum subsp. carotovorum Pcc21 and Reduces Soft-Rot Disease.

Yu S, Kang J, Chung E, Lee Y Plant Pathol J. 2023; 39(1):62-74.

PMID: 36760050 PMC: 9929172. DOI: 10.5423/PPJ.OA.09.2022.0135.

ATP-Binding Cassette Transporters: Snap-on Complexes?.

Younus I, Kochkina S, Choi C, Sun W, Ford R Subcell Biochem. 2022; 99:35-82.

PMID: 36151373 DOI: 10.1007/978-3-031-00793-4_2.

References

Kneidinger B, Marolda C, Graninger M, Zamyatina A, McArthur F, Kosma P . Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli. J Bacteriol. 2001; 184(2):363-9. PMC: 139585. DOI: 10.1128/JB.184.2.363-369.2002. View

Liu R, Blackwell T, States D . Conformational model for binding site recognition by the E.coli MetJ transcription factor. Bioinformatics. 2001; 17(7):622-33. DOI: 10.1093/bioinformatics/17.7.622. View

Kadner R . Transport systems for L-methionine in Escherichia coli. J Bacteriol. 1974; 117(1):232-41. PMC: 246549. DOI: 10.1128/jb.117.1.232-241.1974. View

Kadner R, Watson W . Methionine transport in Escherichia coli: physiological and genetic evidence for two uptake systems. J Bacteriol. 1974; 119(2):401-9. PMC: 245621. DOI: 10.1128/jb.119.2.401-409.1974. View

Kadner R . Regulation of methionine transport activity in Escherichia coli. J Bacteriol. 1975; 122(1):110-9. PMC: 235647. DOI: 10.1128/jb.122.1.110-119.1975. View

Kadner R, Winkler H . Energy coupling for methionine transport in Escherichia coli. J Bacteriol. 1975; 123(3):985-91. PMC: 235823. DOI: 10.1128/jb.123.3.985-991.1975. View

Kadner R . Transport and utilization of D-methionine and other methionine sources in Escherichia coli. J Bacteriol. 1977; 129(1):207-16. PMC: 234917. DOI: 10.1128/jb.129.1.207-216.1977. View

Masters M . The frequency of P1 transduction of the genes of Escherichia coli as a function of chromosomal position: preferential transduction of the origin of replication. Mol Gen Genet. 1977; 155(2):197-202. DOI: 10.1007/BF00393160. View

Bohman K, ISAKSSON L . A temperature-sensitive mutant in prolinyl-tRNA ligase of Escherichia coli K-12. Mol Gen Genet. 1980; 177(4):603-5. DOI: 10.1007/BF00272670. View

10.

Ellwood M, Nomura M . Chromosomal locations of the genes for rRNA in Escherichia coli K-12. J Bacteriol. 1982; 149(2):458-68. PMC: 216529. DOI: 10.1128/jb.149.2.458-468.1982. View

11.

Cottam A, Ayling P . Genetic studies of mutants in a high-affinity methionine transport system in Salmonella typhimurium. Mol Gen Genet. 1989; 215(2):358-63. DOI: 10.1007/BF00339743. View

12.

Grundy C, Ayling P . Fine structure mapping and complementation studies of the metD methionine transport system in Salmonella typhimurium. Genet Res. 1992; 60(1):1-6. DOI: 10.1017/s0016672300030603. View

13.

Cherepanov P, Wackernagel W . Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene. 1995; 158(1):9-14. DOI: 10.1016/0378-1119(95)00193-a. View

14.

Blattner F, Plunkett 3rd G, Bloch C, Perna N, Burland V, Riley M . The complete genome sequence of Escherichia coli K-12. Science. 1997; 277(5331):1453-62. DOI: 10.1126/science.277.5331.1453. View

15.

Paulsen I, Sliwinski M, Saier Jr M . Microbial genome analyses: global comparisons of transport capabilities based on phylogenies, bioenergetics and substrate specificities. J Mol Biol. 1998; 277(3):573-92. DOI: 10.1006/jmbi.1998.1609. View

16.

Berlyn M . Linkage map of Escherichia coli K-12, edition 10: the traditional map. Microbiol Mol Biol Rev. 1998; 62(3):814-984. PMC: 98936. DOI: 10.1128/MMBR.62.3.814-984.1998. View

17.

Nichols B, Shafiq O, Meiners V . Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J Bacteriol. 1998; 180(23):6408-11. PMC: 107733. DOI: 10.1128/JB.180.23.6408-6411.1998. View

18.

Jones P, George A . Subunit interactions in ABC transporters: towards a functional architecture. FEMS Microbiol Lett. 1999; 179(2):187-202. DOI: 10.1111/j.1574-6968.1999.tb08727.x. View

19.

Braibant M, Gilot P, Content J . The ATP binding cassette (ABC) transport systems of Mycobacterium tuberculosis. FEMS Microbiol Rev. 2000; 24(4):449-67. DOI: 10.1111/j.1574-6976.2000.tb00550.x. View

20.

Merlin C, McAteer S, Masters M . Tools for characterization of Escherichia coli genes of unknown function. J Bacteriol. 2002; 184(16):4573-81. PMC: 135234. DOI: 10.1128/JB.184.16.4573-4581.2002. View