Development and Distribution of the Peptidergic System in Larval and Adult Patiriella: Comparison of Sea Star Bilateral and Radial Nervous Systems

Development of the larval peptidergic system in the sea star Patiriella regularis and structure of the adult nervous system in Patiriella species were documented in an immunofluorescence investigation using antisera to the sea star neuropeptide GFNSALMFamide 1 (S1) and confocal microscopy. P. regularis has planktotrophic development through bipinnaria and brachiolaria larvae. In early bipinnaria, two groups of immunoreactive cells appeared on either side of the anterior region and proliferated to form a pair of dorsolateral ganglia. The ganglia gave rise to fine varicose fibres that innervated the preoral and adoral ciliated bands. Peptidergic cells also innervated the postoral ciliated band, and a nerve tract connected the pre- and postoral bands. Fully developed bipinnaria had a well-developed peptidergic system, the organisation of which reflected the bilateral larval body plan. As the brachiolar attachment complex differentiated at the anterior end, the ganglia became positioned on either side of the anterior projection, from which they innervated the complex. It is suggested, based on the distribution of S1-like immunoreactivity in association with ciliary and attachment structures, that the peptidergic system functions in modulation of feeding, swimming, and settlement. The larval peptidergic system degenerates as the larval body is resorbed during metamorphosis. In adults, S1-like immunoreactivity was intense in the axonal region of the ectoneural nervous system and in hyponeural perikarya. Immunoreactive cells in the neuroepithelium connected with the surface and may be sensory. Examination of immunoreactivity in several Patiriella species attests to the highly conserved organisation of the peptidergic system in adult asteroids.