Comparison of Serological Detection Methods for Diagnosis of Ehrlichia Canis Infections in Dogs

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2002 Aug 31

PMID 12202605

Citations 8

Authors

Myriam Belanger

Heather L Sorenson

Michelle K France

Michael V Bowie

Anthony F Barbet

Edward B Breitschwerdt

A Rick Alleman

Affiliations

Soon will be listed here.

Abstract

We determined the value of four serological assays for the diagnosis of canine monocytic ehrlichiosis by comparing them to the indirect fluorescent-antibody assay "gold standard." The specificity of Dip-S-Ticks was significantly lower than that of all of the other tests evaluated. The sensitivity of Dip-S-Ticks was significantly higher than that of Snap3Dx or the Snap Canine Combo. The sensitivity of the rMAP2 enzyme-linked immunosorbent assay (ELISA) was significantly higher than that of the Snap Canine Combo. The accuracy levels of the rMAP2 ELISA, Snap3Dx, Dip-S-Ticks, and Snap Canine Combo were 97.0, 89.8, 85.1, and 82.9%, respectively.

Citing Articles

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References

Chen S, Cullman L, Walker D . Western immunoblotting analysis of the antibody responses of patients with human monocytotropic ehrlichiosis to different strains of Ehrlichia chaffeensis and Ehrlichia canis. Clin Diagn Lab Immunol. 1998; 4(6):731-5. PMC: 170650. DOI: 10.1128/cdli.4.6.731-735.1997. View

Breitschwerdt E, Hegarty B, Hancock S . Doxycycline hyclate treatment of experimental canine ehrlichiosis followed by challenge inoculation with two Ehrlichia canis strains. Antimicrob Agents Chemother. 1998; 42(2):362-8. PMC: 105415. DOI: 10.1128/AAC.42.2.362. View

Reddy G, Sulsona C, Barbet A, Mahan S, Burridge M, Alleman A . Molecular characterization of a 28 kDa surface antigen gene family of the tribe Ehrlichiae. Biochem Biophys Res Commun. 1998; 247(3):636-43. DOI: 10.1006/bbrc.1998.8844. View

Breitschwerdt E, Hegarty B, Hancock S . Sequential evaluation of dogs naturally infected with Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia equi, Ehrlichia ewingii, or Bartonella vinsonii. J Clin Microbiol. 1998; 36(9):2645-51. PMC: 105178. DOI: 10.1128/JCM.36.9.2645-2651.1998. View

Ohashi N, Unver A, Zhi N, Rikihisa Y . Cloning and characterization of multigenes encoding the immunodominant 30-kilodalton major outer membrane proteins of Ehrlichia canis and application of the recombinant protein for serodiagnosis. J Clin Microbiol. 1998; 36(9):2671-80. PMC: 105182. DOI: 10.1128/JCM.36.9.2671-2680.1998. View

Kordick S, Breitschwerdt E, Hegarty B, Southwick K, Colitz C, Hancock S . Coinfection with multiple tick-borne pathogens in a Walker Hound kennel in North Carolina. J Clin Microbiol. 1999; 37(8):2631-8. PMC: 85300. DOI: 10.1128/JCM.37.8.2631-2638.1999. View

Rikihisa Y, Ewing S, Fox J . Western immunoblot analysis of Ehrlichia chaffeensis, E. canis, or E. ewingii infections in dogs and humans. J Clin Microbiol. 1994; 32(9):2107-12. PMC: 263951. DOI: 10.1128/jcm.32.9.2107-2112.1994. View

Waner T, Strenger C, Keysary A . Comparison of a clinic-based ELISA test kit with the immunofluorescence test for the assay of Ehrlichia canis antibodies in dogs. J Vet Diagn Invest. 2000; 12(3):240-4. DOI: 10.1177/104063870001200307. View

McBride J, Corstvet R, Breitschwerdt E, Walker D . Immunodiagnosis of Ehrlichia canis infection with recombinant proteins. J Clin Microbiol. 2001; 39(1):315-22. PMC: 87721. DOI: 10.1128/JCM.39.1.315-322.2001. View

10.

Waner T, Harrus S, Jongejan F, Bark H, Keysary A, Cornelissen A . Significance of serological testing for ehrlichial diseases in dogs with special emphasis on the diagnosis of canine monocytic ehrlichiosis caused by Ehrlichia canis. Vet Parasitol. 2001; 95(1):1-15. DOI: 10.1016/s0304-4017(00)00407-6. View

11.

Alleman A, McSherry L, Barbet A, Breitschwerdt E, Sorenson H, Bowie M . Recombinant major antigenic protein 2 of Ehrlichia canis: a potential diagnostic tool. J Clin Microbiol. 2001; 39(7):2494-9. PMC: 88175. DOI: 10.1128/JCM.39.7.2494-2499.2001. View

12.

Courtney C, CORNELL J . Evaluation of heartworm immunodiagnostic tests. J Am Vet Med Assoc. 1990; 197(6):724-9. View

13.

Suksawat J, Hegarty B, Breitschwerdt E . Seroprevalence of Ehrlichia canis, Ehrlichia equi, and Ehrlichia risticii in sick dogs from North Carolina and Virginia. J Vet Intern Med. 2000; 14(1):50-5. DOI: 10.1892/0891-6640(2000)014<0050:socear>2.3.co;2. View