Rab3D is Not Required for Exocrine Exocytosis but for Maintenance of Normally Sized Secretory Granules

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2002 Aug 23

PMID 12192047

Citations 62

Authors

Dietmar Riedel

Wolfram Antonin

Rafael Fernandez-Chacon

Guillermo Alvarez de Toledo

Tobias Jo

Martin Geppert

Jack A Valentijn

Karin Valentijn

James D Jamieson

Thomas C Sudhof

Reinhard Jahn

Affiliations

Soon will be listed here.

Abstract

Rab3D, a member of the Rab3 subfamily of the Rab/ypt GTPases, is expressed on zymogen granules in the pancreas as well as on secretory vesicles in mast cells and in the parotid gland. To shed light on the function of Rab3D, we have generated Rab3D-deficient mice. These mice are viable and have no obvious phenotypic changes. Secretion of mast cells is normal as revealed by capacitance patch clamping. Furthermore, enzyme content and overall morphology are unchanged in pancreatic and parotid acinar cells of knockout mice. Both the exocrine pancreas and the parotid gland show normal release kinetics in response to secretagogue stimulation, suggesting that Rab3D is not involved in exocytosis. However, the size of secretory granules in both the exocrine pancreas and the parotid gland is significantly increased, with the volume being doubled. We conclude that Rab3D exerts its function during granule maturation, possibly by preventing homotypic fusion of secretory granules.

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