A Technique for in Situ Karyotyping of Primary Amniotic Fluid Cell Cultures

Overview

Journal Humangenetik

Specialty Genetics

Date 1975 Dec 23

PMID 1218862

Citations 13

Authors

W Schmid

Affiliations

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Abstract

A time proven technique is described for growing amniotic fluid cell cultures on cover glasses in Leighton tubes and for processing the mitotic cells in situ. Karyotyping the clones in situ eliminates most of the problems caused by somatic chromosome mutations in vitro and by maternal cell growth.

Citing Articles

Asymmetric aneuploidy in mesenchymal stromal cells detected by in situ karyotyping and fluorescence in situ hybridization: suggestions for reference values for stem cells.

Kim S, Im K, Park S, Kwon J, Kim J, Choi Q Stem Cells Dev. 2014; 24(1):77-92.

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The application of an in situ karyotyping technique for mesenchymal stromal cells: a validation and comparison study with classical G-banding.

Hwang S, See C, Choi J, Kim S, Choi Q, Kim J Exp Mol Med. 2013; 45:e68.

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Effects of radical-scavenging enzymes and reduced oxygen exposure on growth and chromosome abnormalities of Werner syndrome cultured skin fibroblasts.

Salk D, Au K, Hoehn H, Martin G Hum Genet. 1981; 57(3):269-75.

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Incomplete trisomy 22. III. Mosaic-trisomy 22 and the problem of full trisomy 22.

Schinzel A Hum Genet. 1981; 56(3):269-73.

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The offspring of marriage between two first cousins with the same reciprocal translocation t(2;7)(p11;q31).

Simoni G, Dalpra L, Terzoli G, Rossella F, Tibiletti M Hum Genet. 1980; 55(2):199-202.

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