Effects of Polyamines and Methylglyoxal Bis(guanylhydrazone) on Hepatic Nuclear Structure and Deoxyribonucleic Acid Template Activity

Overview

Journal Biochem J

Specialty Biochemistry

Date 1975 Dec 1

PMID 1218090

Citations 3

Authors

K B Brown

N F Nelson

D G Brown

Affiliations

Soon will be listed here.

Abstract

1. The interaction of polyamines and methylglyoxal bis(guanythydrazone) (1, 1'-[(methylethanediylidene)-dinitrilo]diguanidine) with isolated rat liver nuclei was investigated by electron microscopy. 2. At 4mM, putrescine was without effect; however, spermidine, spermine or methylglyoxal bis(guanythydrazone) resulted in dispersed chromatin and alterations in nucleolar structure. In addition, spermidine or methylglyoxal bis(guanylhydrazone) caused marked aggregation of interchromatin granules. 3. The DNA template property of calf thymus DNA was examined by using DNA polymerases from Escherichia coli, Micrococcus lysodeikticus and calf thymus in the presence of 0-5 mM-amine. 4. In the presence of DNA polymerase, spermine or methylglyoxal bis(guanylhydrazone) inhibited activity, whereas putrescine or spermidine had much less effect or in some cases stimulated [3H]dTMP incorporation. 5. Template activity which was inhibited by spermine or methylglyoxal bis(guanylhydrazone) could be partially restored by additional DNA or enzyme. 6. When mixed with calf thymus DNA, calf thymus histone inhibited template activity as measured with E. coli DNA polymerase. The template activity of such a 'histone-nucleate' could not be restored by putrescine, spermidine, spermine or methylglyoxal bis(guanylhydrazone). 7. DNA template activity of isolated rat liver nuclei was tested by using E. coli DNA polymerase. None of the amines was able to increase the template activity of the nuclear DNA in vitro.

Citing Articles

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Irreversible inhibition of putrescine-stimulated S-adenosyl-L-methionine decarboxylase by berenil and pentamidine.

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Polyamine induced aggregation of DNA.

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References

AGRELL I, Heby O . Interaction between spermine and histones at nucleoprotein formation. Hoppe Seylers Z Physiol Chem. 1971; 352(1):39-42. DOI: 10.1515/bchm2.1971.352.1.39. View

HIRSCHMAN S, Leng M, Felsenfield G . Interaction of spermine and DNA. Biopolymers. 1967; 5(2):227-33. DOI: 10.1002/bip.1967.360050209. View

Brown D, Coffey D . The release of chromatin template restrictions by natural polyribonucleotides. Biochem Biophys Res Commun. 1971; 42(2):326-33. DOI: 10.1016/0006-291x(71)90106-9. View

WILLIAMS-ASHMAN H, Schenone A . Methyl glyoxal bis(guanylhydrazone) as a potent inhibitor of mammalian and yeast S-adenosylmethionine decarboxylases. Biochem Biophys Res Commun. 1972; 46(1):288-95. DOI: 10.1016/0006-291x(72)90661-4. View

Corti A, Dave C, WILLIAMS-ASHMAN H, Mihich E, Schenone A . Specific inhibition of the enzymic decarboxylation of S-adenosylmethionine by methylglyoxal bis(guanylhydrazone) and related substances. Biochem J. 1974; 139(2):351-7. PMC: 1166290. DOI: 10.1042/bj1390351. View

Heby O, Sauter S, Russell D . Stimulation of ornithine decarboxylase activity and inhibition of S-adenosyl-L-methionine decarboxylase activity in leukaemic mice by methylglyoxal bis(guanylhydrazone). Biochem J. 1973; 136(4):1121-4. PMC: 1166065. DOI: 10.1042/bj1361121. View

Pegg A, Corti A, WILLIAMS-ASHMAN H . Paradoxical enhancement of S-adenosylmethionine decarboxylase in rat tissues following administration of the specific inhibitor methyl glyoxal bis(guanylhydrazone). Biochem Biophys Res Commun. 1973; 52(2):696-701. DOI: 10.1016/0006-291x(73)90768-7. View

Russell D . Increased polyamine concentrations in the urine of human cancer patients. Nat New Biol. 1971; 233(39):144-5. DOI: 10.1038/newbio233144a0. View

Sartorelli A, IANNOTTI A, BOOTH B, SCHNEIDER F, Bertino J, Johns D . COMPLEX FORMATION WITH DNA AND INHIBITION OF NUCLEIC ACID SYNTHESIS BY METHYLGLYOXAL BIS(GUANYLHYDRAZONE). Biochim Biophys Acta. 1965; 103:174-6. DOI: 10.1016/0005-2787(65)90550-2. View

10.

Pegg A . Inhibition of spermidine formation in rat liver and kidney by methylglyoxal bis(guanylhydrazone). Biochem J. 1973; 132(3):537-40. PMC: 1177618. DOI: 10.1042/bj1320537. View

11.

Stevens L . The biochemical role of naturally occurring polyamines in nucleic acid synthesis. Biol Rev Camb Philos Soc. 1970; 45(1):1-27. DOI: 10.1111/j.1469-185x.1970.tb01073.x. View

12.

Mihich E . CURRENT STUDIES WITH METHYLGLYOXAL-BIS(GUANYLHYDRAZONE). Cancer Res. 1963; 23:1375-89. View

13.

Marton L, Heby O, Wilson C . Increased polyamine concentrations in the cerebrospinal fluid of patients with brain tumors. Int J Cancer. 1974; 14(6):731-5. DOI: 10.1002/ijc.2910140606. View

14.

Gfeller E, STERN D, Russell D, Levy C, Taylor R . Ultrastructural changes in vitro of rat liver nucleoli in response to polyamines. Z Zellforsch Mikrosk Anat. 1972; 129(4):447-54. DOI: 10.1007/BF00316742. View

15.

Brown D, Coffey D . Effects of polyinosinic acid and polycytidylic acid on the deoxyribonucleic acid template activity of isolated nuclei and soluble chromatin from rat liver. J Biol Chem. 1972; 247(23):7674-83. View

16.

OBRIEN R, OLENICK J, Hahn F . Reactions of quinine, chloroquine, and quinacrine with DNA and their effects on the DNA and RNA polymerase reactions. Proc Natl Acad Sci U S A. 1966; 55(6):1511-7. PMC: 224352. DOI: 10.1073/pnas.55.6.1511. View

17.

Arnold E, Yawn D, Brown D, Wyllie R, Coffey D . Structural alteration in isolated rat liver nuclei after removal of template restriction by polyanions. J Cell Biol. 1972; 53(3):737-57. PMC: 2108763. DOI: 10.1083/jcb.53.3.737. View

18.

Chiu J, Sung S . Effect of spermidine on the activity of DNA polymerases. Biochim Biophys Acta. 1972; 281(4):535-42. DOI: 10.1016/0005-2787(72)90154-2. View

19.

Snyder S, Russell D . Polyamine synthesis in rapidly growing tissues. Fed Proc. 1970; 29(4):1575-82. View

20.

Blobel G, POTTER V . Nuclei from rat liver: isolation method that combines purity with high yield. Science. 1966; 154(3757):1662-5. DOI: 10.1126/science.154.3757.1662. View