Plant Colonization by the Vascular Wilt Fungus Fusarium Oxysporum Requires FOW1, a Gene Encoding a Mitochondrial Protein

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 2002 Aug 13

PMID 12172028

Citations 45

Authors

Iori Inoue

Fumio Namiki

Takashi Tsuge

Affiliations

Soon will be listed here.

Abstract

The soil-borne fungus Fusarium oxysporum causes vascular wilts of a wide variety of plant species by directly penetrating roots and colonizing the vascular tissue. The pathogenicity mutant B60 of the melon wilt pathogen F. oxysporum f. sp. melonis was isolated previously by restriction enzyme-mediated DNA integration mutagenesis. Molecular analysis of B60 identified the affected gene, designated FOW1, which encodes a protein with strong similarity to mitochondrial carrier proteins of yeast. Although the FOW1 insertional mutant and gene-targeted mutants showed normal growth and conidiation in culture, they showed markedly reduced virulence as a result of a defect in the ability to colonize the plant tissue. Mitochondrial import of Fow1 was verified using strains expressing the Fow1-green fluorescent protein fusion proteins. The FOW1-targeted mutants of the tomato wilt pathogen F. oxysporum f. sp. lycopersici also showed reduced virulence. These data strongly suggest that FOW1 encodes a mitochondrial carrier protein that is required specifically for colonization in the plant tissue by F. oxysporum.

Citing Articles

The Ubiquitous Wilt-Inducing Pathogen -A Review of Genes Studied with Mutant Analysis.

Jackson E, Li J, Weerasinghe T, Li X Pathogens. 2024; 13(10).

PMID: 39452695 PMC: 11510031. DOI: 10.3390/pathogens13100823.

Epidemiology and management of Fusarium wilt of through systemic acquired resistance.

Ahmad I, Mazhar K, Atiq M, Khalaf A, Rashid M, Asif M PeerJ. 2024; 12:e17022.

PMID: 38563017 PMC: 10984168. DOI: 10.7717/peerj.17022.

Histopathology and quantification of green fluorescent protein-tagged f. sp. isolate in resistant and susceptible germplasm.

Namisy A, Chen S, Huang J, Unartngam J, Thanarut C, Chung W Microbiol Spectr. 2024; 12(2):e0312723.

PMID: 38174927 PMC: 10846128. DOI: 10.1128/spectrum.03127-23.

Mitonuclear Genetic Interactions in the Basidiomycete Involve a Non-conserved Mitochondrial Open Reading Frame.

Clergeot P, Olson A Front Fungal Biol. 2023; 2:779337.

PMID: 37744141 PMC: 10512249. DOI: 10.3389/ffunb.2021.779337.

Characterization of Three spp. Causing Wilt Disease of L. in Korea.

Koo Y, Ahsan S, Choi H Mycobiology. 2023; 51(3):186-194.

PMID: 37359955 PMC: 10288908. DOI: 10.1080/12298093.2023.2213911.

References

Okuda M, Ikeda K, Namiki F, Nishi K, Tsuge T . Tfo1: an Ac-like transposon from the plant pathogenic fungus Fusarium oxysporum. Mol Gen Genet. 1998; 258(6):599-607. DOI: 10.1007/s004380050773. View

Vanden Wymelenberg A, Cullen D, Spear R, Schoenike B, Andrews J . Expression of green fluorescent protein in Aureobasidium pullulans and quantification of the fungus on leaf surfaces. Biotechniques. 1997; 23(4):686-90. DOI: 10.2144/97234st01. View

Mayor J, Kakhniashvili D, Gremse D, Campbell C, Kramer R, Schroers A . Bacterial overexpression of putative yeast mitochondrial transport proteins. J Bioenerg Biomembr. 1998; 29(6):541-7. DOI: 10.1023/a:1022426900735. View

Li D, Rogers L, Kolattukudy P . Cloning and expression of cDNA encoding a mitogen-activated protein kinase from a phytopathogenic filamentous fungus. Gene. 1997; 195(2):161-6. DOI: 10.1016/s0378-1119(97)00124-8. View

Idnurm A, Howlett B . Pathogenicity genes of phytopathogenic fungi. Mol Plant Pathol. 2010; 2(4):241-55. DOI: 10.1046/j.1464-6722.2001.00070.x. View

Tzagoloff A, Dieckmann C . PET genes of Saccharomyces cerevisiae. Microbiol Rev. 1990; 54(3):211-25. PMC: 372773. DOI: 10.1128/mr.54.3.211-225.1990. View

Palmieri L, Runswick M, Fiermonte G, Walker J, Palmieri F . Yeast mitochondrial carriers: bacterial expression, biochemical identification and metabolic significance. J Bioenerg Biomembr. 2002; 32(1):67-77. DOI: 10.1023/a:1005564429242. View

Xu J . Map kinases in fungal pathogens. Fungal Genet Biol. 2001; 31(3):137-52. DOI: 10.1006/fgbi.2000.1237. View

Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W . Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25(17):3389-402. PMC: 146917. DOI: 10.1093/nar/25.17.3389. View

10.

Namiki F, Shiomi T, Nishi K, Kayamura T, Tsuge T . Pathogenic and Genetic Variation in the Japanese Strains of Fusarium oxysporum f. sp. melonis. Phytopathology. 2008; 88(8):804-10. DOI: 10.1094/PHYTO.1998.88.8.804. View

11.

Kimura A, Takano Y, Furusawa I, Okuno T . Peroxisomal metabolic function is required for appressorium-mediated plant infection by Colletotrichum lagenarium. Plant Cell. 2001; 13(8):1945-57. PMC: 139132. DOI: 10.1105/tpc.010084. View

12.

Oliver R, Osbourn A . Molecular dissection of fungal phytopathogenicity. Microbiology (Reading). 1995; 141 ( Pt 1):1-9. DOI: 10.1099/00221287-141-1-1. View

13.

Knogge W . Fungal pathogenicity. Curr Opin Plant Biol. 1999; 1(4):324-8. DOI: 10.1016/1369-5266(88)80054-2. View

14.

Nelson D, Felix C, Swanson J . Highly conserved charge-pair networks in the mitochondrial carrier family. J Mol Biol. 1998; 277(2):285-308. DOI: 10.1006/jmbi.1997.1594. View

15.

Mullaney E, Hamer J, Roberti K, Yelton M, Timberlake W . Primary structure of the trpC gene from Aspergillus nidulans. Mol Gen Genet. 1985; 199(1):37-45. DOI: 10.1007/BF00327506. View

16.

Namiki F, Shiomi T, Kayamura T, Tsuge T . Characterization of the formae speciales of Fusarium oxysporum causing wilts of cucurbits by DNA fingerprinting with nuclear repetitive DNA sequences. Appl Environ Microbiol. 1994; 60(8):2684-91. PMC: 201709. DOI: 10.1128/aem.60.8.2684-2691.1994. View

17.

di Pietro A, Garcia-Maceira F, Meglecz E, Roncero M . A MAP kinase of the vascular wilt fungus Fusarium oxysporum is essential for root penetration and pathogenesis. Mol Microbiol. 2001; 39(5):1140-52. View

18.

Belenkiy R, Haefele A, Eisen M, WOHLRAB H . The yeast mitochondrial transport proteins: new sequences and consensus residues, lack of direct relation between consensus residues and transmembrane helices, expression patterns of the transport protein genes, and protein-protein interactions with.... Biochim Biophys Acta. 2000; 1467(1):207-18. DOI: 10.1016/s0005-2736(00)00222-4. View

19.

Mendgen K . Cell wall synthesis in cotton roots after infection with Fusarium oxysporum. The deposition of callose, arabinogalactans, xyloglucans, and pectic components into walls, wall appositions, cell plates and plasmodesmata. Planta. 1995; 197(3):535-45. DOI: 10.1007/BF00196676. View

20.

Roldan-Arjona T, Perez-Espinosa A, Ruiz-Rubio M . Tomatinase from Fusarium oxysporum f. sp. lycopersici defines a new class of saponinases. Mol Plant Microbe Interact. 1999; 12(10):852-61. DOI: 10.1094/MPMI.1999.12.10.852. View