Determination of Mitochondrial Reactive Oxygen Species: Methodological Aspects

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2002 Aug 10

PMID 12169203

Citations 43

Authors

Cecile Batandier

E Fontaine

Christiane Keriel

X M Leverve

Affiliations

Soon will be listed here.

Abstract

The generation of Reactive Oxygen Species (ROS) as by-products in mitochondria Electron Transport Chain (ETC) has long been admitted as the cost of aerobic energy metabolism with oxidative damages as consequence. The purpose of this methodological review is to present some of the most widespread methods of ROS generation and to underline the limitations as well as some problems, identified with some experiments as examples, in the interpretation of such results. There is now no doubt that besides their pejorative role, ROS are involved in a variety of cellular processes for the continuous adaptation of the cell to its environment. Because ROS metabolism is a complex area (low production, instability of species, efficient antioxidant defense system, several places of production...) bias, variances and limitations in ROS measurements must be recognized in order to avoid artefactual conclusions, and especially to improve our understanding of physiological and pathophysiological mechanisms of such phenomenon.

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References

Raisanen S, Lehenkari P, Tasanen M, Rahkila P, Harkonen P, Vaananen H . Carbonic anhydrase III protects cells from hydrogen peroxide-induced apoptosis. FASEB J. 1999; 13(3):513-22. DOI: 10.1096/fasebj.13.3.513. View

Dalton T, Shertzer H, Puga A . Regulation of gene expression by reactive oxygen. Annu Rev Pharmacol Toxicol. 1999; 39:67-101. DOI: 10.1146/annurev.pharmtox.39.1.67. View

Bejma J, Ji L . Aging and acute exercise enhance free radical generation in rat skeletal muscle. J Appl Physiol (1985). 1999; 87(1):465-70. DOI: 10.1152/jappl.1999.87.1.465. View

Hempel S, Buettner G, OMalley Y, Wessels D, Flaherty D . Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med. 1999; 27(1-2):146-59. DOI: 10.1016/s0891-5849(99)00061-1. View

Li Y, Stansbury K, Zhu H, Trush M . Biochemical characterization of lucigenin (Bis-N-methylacridinium) as a chemiluminescent probe for detecting intramitochondrial superoxide anion radical production. Biochem Biophys Res Commun. 1999; 262(1):80-7. DOI: 10.1006/bbrc.1999.1174. View

Chen Y, Sturgeon B, Gunther M, Mason R . Electron spin resonance investigation of the cyanyl and azidyl radical formation by cytochrome c oxidase. J Biol Chem. 1999; 274(35):24611-6. DOI: 10.1074/jbc.274.35.24611. View

Pansarasa O, Bertorelli L, Vecchiet J, Felzani G, Marzatico F . Age-dependent changes of antioxidant activities and markers of free radical damage in human skeletal muscle. Free Radic Biol Med. 1999; 27(5-6):617-22. DOI: 10.1016/s0891-5849(99)00108-2. View

Rota C, Fann Y, Mason R . Phenoxyl free radical formation during the oxidation of the fluorescent dye 2',7'-dichlorofluorescein by horseradish peroxidase. Possible consequences for oxidative stress measurements. J Biol Chem. 1999; 274(40):28161-8. DOI: 10.1074/jbc.274.40.28161. View

Esposti M, Hatzinisiriou I, McLennan H, Ralph S . Bcl-2 and mitochondrial oxygen radicals. New approaches with reactive oxygen species-sensitive probes. J Biol Chem. 1999; 274(42):29831-7. DOI: 10.1074/jbc.274.42.29831. View

10.

Staniek K, Nohl H . H(2)O(2) detection from intact mitochondria as a measure for one-electron reduction of dioxygen requires a non-invasive assay system. Biochim Biophys Acta. 1999; 1413(2):70-80. DOI: 10.1016/s0005-2728(99)00083-3. View

11.

Halliwell B . Antioxidant defence mechanisms: from the beginning to the end (of the beginning). Free Radic Res. 1999; 31(4):261-72. DOI: 10.1080/10715769900300841. View

12.

Sureda F, Gabriel C, Comas J, Pallas M, Escubedo E, Camarasa J . Evaluation of free radical production, mitochondrial membrane potential and cytoplasmic calcium in mammalian neurons by flow cytometry. Brain Res Brain Res Protoc. 1999; 4(3):280-7. DOI: 10.1016/s1385-299x(99)00030-6. View

13.

Cai J, Jones D . Mitochondrial redox signaling during apoptosis. J Bioenerg Biomembr. 2000; 31(4):327-34. DOI: 10.1023/a:1005423818280. View

14.

Barja G . Mitochondrial oxygen radical generation and leak: sites of production in states 4 and 3, organ specificity, and relation to aging and longevity. J Bioenerg Biomembr. 2000; 31(4):347-66. DOI: 10.1023/a:1005427919188. View

15.

Fridovich I . Fundamental aspects of reactive oxygen species, or what's the matter with oxygen?. Ann N Y Acad Sci. 2000; 893:13-8. DOI: 10.1111/j.1749-6632.1999.tb07814.x. View

16.

Chandel N, Schumacker P . Cellular oxygen sensing by mitochondria: old questions, new insight. J Appl Physiol (1985). 2000; 88(5):1880-9. DOI: 10.1152/jappl.2000.88.5.1880. View

17.

Lee H, Yin P, Lu C, Chi C, Wei Y . Increase of mitochondria and mitochondrial DNA in response to oxidative stress in human cells. Biochem J. 2000; 348 Pt 2:425-32. PMC: 1221082. View

18.

Antunes F, Cadenas E . Estimation of H2O2 gradients across biomembranes. FEBS Lett. 2000; 475(2):121-6. DOI: 10.1016/s0014-5793(00)01638-0. View

19.

Skulachev V . Mitochondria in the programmed death phenomena; a principle of biology: "it is better to die than to be wrong". IUBMB Life. 2000; 49(5):365-73. DOI: 10.1080/152165400410209. View

20.

Melov S . Mitochondrial oxidative stress. Physiologic consequences and potential for a role in aging. Ann N Y Acad Sci. 2000; 908:219-25. DOI: 10.1111/j.1749-6632.2000.tb06649.x. View