A New Promoter for Alpha1C Subunit of Human L-type Cardiac Calcium Channel Ca(V)1.2

Overview

Journal Biochem Biophys Res Commun

Publisher Elsevier

Specialty Biochemistry

Date 2002 Aug 7

PMID 12163037

Citations 16

Authors

Bosong Dai

Nehad Saada

Clement Echetebu

Christine Dettbarn

Philip Palade

Affiliations

Soon will be listed here.

Abstract

The cardiac Ca channel known as alpha1C or Ca(V)1.2 is shown to express a new longer first exon equivalent to that formerly reported in rabbit heart or rat aorta. Ribonuclease protection assay indicates that this exon is found in the majority of Ca(V)1.2 transcripts in human heart RNA. The presence of this exon also suggests that expression of this transcript is driven by a promoter immediately upstream of this exon and its 5' untranslated region. The putative promoter exhibits 69% homology to its rat counterpart and displays functional promoter activity when transfected into heart cells in culture in luciferase-expressing constructs.

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