Nucleic Acid Metabolism in Yeast. I. Inhibition of RNA and DNA Synthesis by High Concentrations of Exogenous Deoxythymidine 5'-monophosphate in 5'-dTMP Low Requiring Strains

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1975 Dec 30

PMID 1214779

Citations 5

Authors

U G Langjahr

E M Hartmann

M Brendel

Affiliations

Soon will be listed here.

Abstract

The three haploid yeast strains T2tmpl1-3, T2tmp1-1, and T6tmp1-51 auxotrophic for 5'-dTMP differ in their requirement for thymidylate: 72, 16, and 3 mug 5'-dTMP/ml will restore optimal growth, respectively. Thymidylate low requirement in strain T2tmp1-1 and T6tmp1-51 is termed tlrA and tlrC, respectively. When the growth medium is made 5 x 10(-4) M for 5'-dTMP only strain T6tmp1-51 is severely inhibited in RNA and DNA synthesis. This inhibition is reversible after removal of excessive 5'-dTMP. The inhibitory characteristic is in marked contrast to "thymineless death" due to the lack of 5'-dTMP in strain T6tmp1-51 where only DNA synthesis stops while RNA synthesis continues. The inhibitory effect of 5 x 10(-4) M 5'-dTMP is not due to the 5'-dTMP auxotrophy but to the thymidylate low requiring character (tlrC) in strain T6tmp1-51. The arrest of RNA and DNA synthesis by high concentrations of exogenous 5'-dTMP suggests a regulatory role of either the mono- or triphosphate on nucleoside or nucleotide biosynthesis in yeast.

Citing Articles

Nucleic acid metabolism in yeast II. Metabolism of thymidylate during thymidylate excess death.

Toper R, Fath W, Brendel M Mol Gen Genet. 1981; 182(1):60-4.

PMID: 7022137 DOI: 10.1007/BF00422767.

Nucleic acid metabolism in yeast VI. Utilisation of exogenous dAMP.

Fath W, Brendel M Mol Gen Genet. 1982; 188(1):121-7.

PMID: 6757669 DOI: 10.1007/BF00333005.

Nucleic acid metabolism in yeast, V. Excretion of thymidylate.

Fath W, Brendel M Mol Gen Genet. 1982; 188(1):115-20.

PMID: 6757668 DOI: 10.1007/BF00333004.

Exogenous dTMP utilization by a novel tup mutant of Saccharomyces cerevisiae.

Bisson L, Thorner J J Bacteriol. 1982; 152(1):111-9.

PMID: 6749802 PMC: 221381. DOI: 10.1128/jb.152.1.111-119.1982.

A simple method for the isolation and characterization of thymidylate uptaking mutants in Saccharomyces cerevisiae.

Brendel M Mol Gen Genet. 1976; 147(2):209-15.

PMID: 787759 DOI: 10.1007/BF00267573.

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