Carbamylcholine and Acetylcholine-sensitive, Cation-selective Ionophore As Part of the Purified Acetylcholine Receptor

Overview

Journal J Membr Biol

Date 1975 Dec 4

PMID 1214288

Citations 4

Authors

A E Shamoo

M E Eldefrawi

Affiliations

Soon will be listed here.

Abstract

Black lipid membranes were formed with oxidized cholesterol in the presence of either the acetylcholine receptor, purified from the electric organ of the electric ray Torpedo californica or its tryptic digest. In both cases, conductance of cations increased and was dependent on the concentration of the receptor protein. Conductance of Ca++ was dependent on the concentration, but addition of carbamylcholine gave no reproducible of consistent effects. Only in the case of the tryptic digest of the acetylcholine receptor did carbamylcholine and acetylcholine consistently induce monovalent cation selective conductance (PNa,K: PCl=4.4). The induced monovalent cationic conductance due to carbamylcholine (10 muM) varied from 10- to over 100-fold. Curare (10muM) prevented the action of carbamylcholine. Na-dodecyl sulfate gel electrophoresis of the acetylcholine receptor, before and after tryptic digestion, indicated that this mild enzyme treatment hydrolyzed the receptor molecule subunits. Nevertheless, the receptor molecule retained its full binding of [acetyl(-3)H]acetylcholine; and analytical gel electrophoresis indicated that it remained intact possibly through hydrogen, hydrophobic and disulfice bonding.

Citing Articles

Protease effects on the structure of acetylcholine receptor membranes from Torpedo californica.

Klymkowsky M, Heuser J, Stroud R J Cell Biol. 1980; 85(3):823-38.

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Ca++-induced fusion of fragmented sarcoplasmic reticulum with artificial planar bilayers.

Miller C, RACKER E J Membr Biol. 1976; 30(3):283-300.

PMID: 1009571 DOI: 10.1007/BF01869673.

Biochemical investigations of ionic channels in excitable membranes.

Hucho F, Schiebler W Mol Cell Biochem. 1977; 18(2-3):151-72.

PMID: 342915 DOI: 10.1007/BF00280280.

Perhydrohistrionicotoxin: a potential ligand for the ion conductance modulator of the acetylcholine receptor.

Eldefrawi A, Eldefrawi M, Albuquerque E, Oliveira A, Mansour N, Adler M Proc Natl Acad Sci U S A. 1977; 74(5):2172-6.

PMID: 301278 PMC: 431098. DOI: 10.1073/pnas.74.5.2172.

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