SOX9 Interacts with a Component of the Human Thyroid Hormone Receptor-associated Protein Complex

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2002 Jul 24

PMID 12136106

Citations 35

Authors

Rongjia Zhou

Nathalie Bonneaud

Chao-Xing Yuan

Pascal de Santa Barbara

Brigitte Boizet

Tibor Schomber

Gerd Scherer

Robert G Roeder

Francis Poulat

Philippe Berta

Schomber Tibor

Affiliations

Soon will be listed here.

Abstract

SOX9 transcription factor is involved in chondrocyte differentiation and male sex determination. Heterozygous defects in the human SOX9 gene cause campomelic dysplasia. The mechanisms behind SOX9 function are not understood despite the description of different target genes. This study therefore sets out to identify SOX9-associated proteins to unravel how SOX9 interacts with the cellular transcription machinery. We report the ability of SOX9 to interact with TRAP230, a component of the thyroid hormone receptor-associated protein (TRAP) complex. Both in vitro and in vivo assays have confirmed that the detected interaction is specific and occurs endogenously in cells. Using co-transfection experiments, we have also shown that the TRAP230 interacting domain can act in a dominant-negative manner regarding SOX9 activity. Our results add SOX9 to the list of activators that communicate with the general transcription machinery through the TRAP complex and suggest a basis for the collaboration of SOX9 with different coactivators that could contact the same coactivator/integrator complex.

Citing Articles

Case report: A Non-sense mutation (p.Q417*) located in transactivation domain is Responsible for Campomelic Dysplasia.

Qiao X, Wu L, Tang J, Xiang R, Fan L, Huang H Front Pediatr. 2023; 10:1089194.

PMID: 36741086 PMC: 9890166. DOI: 10.3389/fped.2022.1089194.

SOX9 in organogenesis: shared and unique transcriptional functions.

Ming Z, Vining B, Bagheri-Fam S, Harley V Cell Mol Life Sci. 2022; 79(10):522.

PMID: 36114905 PMC: 9482574. DOI: 10.1007/s00018-022-04543-4.

Hemocyte Clusters Defined by scRNA-Seq in : Analysis of Predicted Marker Genes and Implications for Potential Functional Roles.

Feng M, Swevers L, Sun J Front Immunol. 2022; 13:852702.

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van de Plassche S, Brouwer A Genes (Basel). 2021; 12(5).

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Structure and noncanonical Cdk8 activation mechanism within an Argonaute-containing Mediator kinase module.

Li Y, Chao T, Kim H, Cholko T, Chen S, Li G Sci Adv. 2021; 7(3).

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