CARD In Situ Hybridization: Sights and Signals

Overview

Journal Endocr Pathol

Specialties Endocrinology
Pathology

Date 2002 Jul 13

PMID 12114699

Citations 2

Authors

Ernst J M Speel

Paul Komminoth

Affiliations

Soon will be listed here.

Abstract

During the last decade, several strategies have been developed to improve the detection sensitivity of in situ hybridization (ISV) by amplification of either target nucleic acid sequences prior to ISH (e.g., in situ PCRX or the detection signals after the hybridization procedures (signal amplification). Here we outline the principles of tyramide signal amplification using the catalyzed reporter deposition (CARD) technique, summarize applications as well as possible limitations of CARD 15K, and discuss some future directions of in situ nucleic acid detection using this amplification strategy.

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