Identification of the Enterococcus Faecalis Tyrosine Decarboxylase Operon Involved in Tyramine Production

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2002 Jun 29

PMID 12089039

Citations 43

Authors

Nathalie Connil

Yoann Le Breton

Xavier Dousset

Yanick Auffray

Alain Rince

Herve Prevost

Affiliations

Soon will be listed here.

Abstract

Screening of a library of Enterococcus faecalis insertional mutants allowed isolation of a mutant affected in tyramine production. The growth of this mutant was similar to that of the wild-type E. faecalis JH2-2 strain in Maijala broth, whereas high-performance liquid chromatography analyses showed that tyramine production, which reached 1,000 microg ml(-1) for the wild-type strain, was completely abolished. Genetic analysis of the insertion locus revealed a gene encoding a decarboxylase with similarity to eukaryotic tyrosine decarboxylases. Sequence analysis revealed a pyridoxal phosphate binding site, indicating that this enzyme belongs to the family of amino acid decarboxylases using this cofactor. Reverse transcription-PCR analyses demonstrated that the gene (tdc) encoding the putative tyrosine decarboxylase of E. faecalis JH2-2 is cotranscribed with the downstream gene encoding a putative tyrosine-tyramine antiporter and with the upstream tyrosyl-tRNA synthetase gene. This study is the first description of a tyrosine decarboxylase gene in prokaryotes.

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