Phosphorylation of the Movement Protein of Cucumber Mosaic Virus in Transgenic Tobacco Plants

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 2002 Jun 28

PMID 12086143

Citations 9

Authors

Yasuhiko Matsushita

Kuniaki Yoshioka

Takuma Shigyo

Hideki Takahashi

Hiroshi Nyunoy

Affiliations

Soon will be listed here.

Abstract

The 3a protein of Cucumber mosaic virus is essential for the cell-to-cell movement of the viral RNA through plasmodesmata. We have introduced an epitope peptide before the stop codon of the 3a protein and cloned the tagged ORF into a binary vector for Agrobacterium-mediated transformation. The established transgenic tobacco lines produced the 3a protein, which was specifically detected with anti-3a and anti-epitope antisera. Metabolic labeling and subsequent immunoprecipitation revealed that [32P]-orthophosphate was incorporated into the 3a protein. The phosphoamino acid analysis indicated that the 3a protein contained phosphoserine but not phosphothreonine or phosphotyrosine. This is the first demonstration of the 3a protein phosphorylation in planta.

Citing Articles

The 28 Ser Amino Acid of Cucumber Mosaic Virus Movement Protein Has a Role in Symptom Formation and Plasmodesmata Localization.

Saray R, Fabian A, Palkovics L, Salanki K Viruses. 2021; 13(2).

PMID: 33572676 PMC: 7912182. DOI: 10.3390/v13020222.

Phosphorylations of the Abutilon Mosaic Virus Movement Protein Affect Its Self-Interaction, Symptom Development, Viral DNA Accumulation, and Host Range.

Kleinow T, Happle A, Kober S, Linzmeier L, Rehm T, Fritze J Front Plant Sci. 2020; 11:1155.

PMID: 32849713 PMC: 7411133. DOI: 10.3389/fpls.2020.01155.

Peanut Stunt Virus and Its Satellite RNA Trigger Changes in Phosphorylation in Infected Plants at the Early Stage of the Infection.

Wrzesinska B, Vu L, Gevaert K, De Smet I, Obrepalska-Steplowska A Int J Mol Sci. 2018; 19(10).

PMID: 30340407 PMC: 6214028. DOI: 10.3390/ijms19103223.

Phosphorylation regulates the subcellular localization of Cucumber Mosaic Virus 2b protein.

Nemes K, Gellert A, Almasi A, Vagi P, Saray R, Kadar K Sci Rep. 2017; 7(1):13444.

PMID: 29044170 PMC: 5647415. DOI: 10.1038/s41598-017-13870-7.

Phosphorylation of Beet black scorch virus coat protein by PKA is required for assembly and stability of virus particles.

Zhao X, Wang X, Dong K, Zhang Y, Hu Y, Zhang X Sci Rep. 2015; 5:11585.

PMID: 26108567 PMC: 4479801. DOI: 10.1038/srep11585.

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