Identification of a CDNA Encoding a Plant Lewis-type Alpha1,4-fucosyltransferase

Overview

Journal Glycoconj J

Publisher Springer

Specialties Biochemistry
Endocrinology

Date 2002 Jun 27

PMID 12084979

Citations 8

Authors

I B Wilson

Affiliations

Soon will be listed here.

Abstract

Recently, it has been found that plants, including tomato (Lycopersicon esculentum), express the Lewis-a epitope, Galbeta1,3(Fucalpha1,4)GlcNAc, on some N-glycans. By searching the EST database, it was possible to identify a tomato cDNA encoding a protein, designated FucTC, of 413 amino acids with homology to plant and mammalian alpha1,3/4-fucosyltransferases. The cDNA was expressed in Pichia pastoris and the recombinant enzyme was found to transfer fucose from GDP-Fuc (K(m) 16 microM) to lacto-N-tetraose (Galbeta1,3GlcNAcbeta1,3Galbeta1,4Glc; K(m) 80 microM) as well as to beta1,3- and beta1,4-galactosylated N-glycans. It is concluded that FucTC is responsible for the biosynthesis of Lewis-a on N-glycans in tomato.

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