Transformation of the Edible Mushroom Pleurotus Ostreatus by Particle Bombardment

Overview

Journal FEMS Microbiol Lett

Publisher Oxford University Press

Specialty Microbiology

Date 2002 Jun 22

PMID 12076804

Citations 3

Authors

Masahide Sunagawa

Yumi Magae

Affiliations

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Abstract

Uracil auxotroph of Pleurotus ostreatus was transformed to prototrophy by means of particle bombardment. Five transformants were obtained under three conditions differing in the two parameters of target distance and helium pressure. The transformation frequency was one transformant per microg of DNA. In the transformants, plasmid DNAs were integrated into the genomic DNA and stably maintained. This is the first report on transformation of P. ostreatus by particle bombardment.

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