Transient Expression of the BHLH Factor Neurogenin-2 Marks a Subpopulation of Neural Crest Cells Biased for a Sensory but Not a Neuronal Fate

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2002 Jun 13

PMID 12060754

Citations 62

Authors

Mariela Zirlinger

Liching Lo

Jill McMahon

Andrew P McMahon

David J Anderson

Affiliations

Soon will be listed here.

Abstract

Lineage-tracing experiments have indicated that some premigratory neural crest cells (NCCs) are pleuripotent, generating sensory and sympathetic neurons and their associated glia. Using an inducible Cre recombinase-based fate mapping system, we have permanently marked a subpopulation of NCCs that expresses Ngn2, a bHLH transcription factor required for sensory neurogenesis, and compared its fate to the bulk NCC population marked by expression of Wnt1. Ngn2(+) progenitors were four times more likely than Wnt1(+) NCCs to contribute to sensory rather than sympathetic ganglia. Within dorsal root ganglia, however, both Ngn2- and Wnt1-expressing cells were equally likely to generate neurons or glia. These data suggest that Ngn2 marks an NCC subpopulation with a predictable fate bias, early in migration. Taken together with previous work, these data suggest that NCCs become restricted to sensory or autonomic sublineages before becoming committed to neuronal or glial fates.

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