Human CD34- Hematopoietic Stem Cells: Basic Features and Clinical Relevance

Overview

Journal Int J Hematol

Specialty Hematology

Date 2002 Jun 4

PMID 12041666

Citations 2

Authors

Kiyoshi Ando

Affiliations

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Abstract

CD34 has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+ SCT) has been performed for tumor purging and for prevention of graft-versus-host disease. Recently, CD34-negative hematopoietic stem cells (CD34 HSCs) were identified in mice and humans. Xenogeneic chimera engraftment assays with immunodeficient mice or preimmune fetal sheep resulted in identification of human CD34- HSCs in cord blood, bone marrow, and granulocyte colony-stimulating factor-mobilized peripheral blood, although no significant clonogenic activity was detected in vitro. These characteristics of CD34- HSCs make the assessment of clinical samples difficult. The generation of CD34+ HSCs from CD34 cells in vitro may be a surrogate assay for detecting CD34- HSC activity. This approach was used in recipients of CD34+ SCT and revealed the absence of a CD34 precursor population. The identification of a positive marker in CD34- HSCs and the development of a simpler and more efficient in vivo assay for CD34- HSCs may allow the diagnostic evaluation of human CD34- HSCs in various clinical procedures and diseases.

Citing Articles

Long-term Effects of Regular Whole Blood Donation on Peripheral Blood CD34 Cells Population with using Leukoreduction Filters.

Abbasi Sourki P, Pourfathollah A, Pakjoo M, Abbasi-Malati Z, Tajrishi M Iran J Pathol. 2022; 17(3):335-341.

PMID: 36247501 PMC: 9508535. DOI: 10.30699/IJP.2022.540459.2746.

CD34-negative hematopoietic stem cells show distinct expression profiles of homing molecules that limit engraftment in mice and sheep.

Abe T, Matsuoka Y, Nagao Y, Sonoda Y, Hanazono Y Int J Hematol. 2017; 106(5):631-637.

PMID: 28687990 DOI: 10.1007/s12185-017-2290-5.

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