Ca(2+) Sensor S100beta-modulated Sites of Membrane Guanylate Cyclase in the Photoreceptor-bipolar Synapse

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2002 May 29

PMID 12032068

Citations 35

Authors

Teresa Duda

Karl-Wilhelm Koch

Venkateswar Venkataraman

Christian Lange

Michael Beyermann

Rameshwar K Sharma

Affiliations

Soon will be listed here.

Abstract

This study documents the identity of a calcium- regulated membrane guanylate cyclase transduction system in the photoreceptor-bipolar synaptic region. The guanylate cyclase is the previously characterized ROS-GC1 from the rod outer segments and its modulator is S100beta. S100beta senses increments in free Ca(2+) and stimulates the cyclase. Specificity of photoreceptor guanylate cyclase activation by S100beta is validated by the identification of two S100beta-regulatory sites. A combination of peptide competition, surface plasmon resonance binding and deletion mutation studies has been used to show that these sites are specific for S100beta and not for another regulator of ROS-GC1, guanylate cyclase-activating protein 1. One site comprises amino acids (aa) Gly962-Asn981, the other, aa Ile1030-Gln1041. The former represents the binding site. The latter binds S100beta only marginally, yet it is critical for control of maximal cyclase activity. The findings provide evidence for a new cyclic GMP transduction system in synaptic layers and thereby extend existing concepts of how a membrane-bound guanylate cyclase is regulated by small Ca(2+)-sensor proteins.

Citing Articles

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