Evaluation of Rapid DNA Extraction Methods for the Quantitative Detection of Fungi Using Real-time PCR Analysis

Overview

Journal J Microbiol Methods

Specialty Microbiology

Date 2002 May 29

PMID 12031583

Citations 41

Authors

Richard A Haugland

Nichole Brinkman

Stephen J Vesper

Affiliations

Soon will be listed here.

Abstract

Three comparatively rapid methods for the extraction of DNA from fungal conidia and yeast cells in environmental (air, water and dust) samples were evaluated for use in real-time PCR (TaqMan) analyses. A simple bead milling method was developed to provide sensitive, accurate and precise quantification of target organisms in air and water (tap and surface) samples. However, quantitative analysis of dust samples required further purification of the extracted DNA by a streamlined silica adsorption procedure.

Citing Articles

Determinants of bacterial and fungal microbiota in Finnish home dust: Impact of environmental biodiversity, pets, and occupants.

Hickman B, Kirjavainen P, Taubel M, de Vos W, Salonen A, Korpela K Front Microbiol. 2022; 13:1011521.

PMID: 36419417 PMC: 9676251. DOI: 10.3389/fmicb.2022.1011521.

Development of a Simple DNA Extraction Method and Pan Loop-Mediated Isothermal Amplification Assay for Diagnosis of Candidemia.

Lim D, Jee H, Moon K, Lim C, Jang W Pathogens. 2022; 11(2).

PMID: 35215055 PMC: 8878442. DOI: 10.3390/pathogens11020111.

Vishniacozyma victoriae (syn. Cryptococcus victoriae) in the homes of asthmatic and non-asthmatic children in New York City.

Rush R, Dannemiller K, Cochran S, Haines S, Acosta L, Divjan A J Expo Sci Environ Epidemiol. 2021; 32(1):48-59.

PMID: 34091598 PMC: 10032026. DOI: 10.1038/s41370-021-00342-4.

Incorporating polymerase chain reaction-based identification, population characterization, and quantification of microorganisms into aerosol science: A review.

Peccia J, Hernandez M Atmos Environ (1994). 2020; 40(21):3941-3961.

PMID: 32288550 PMC: 7108281. DOI: 10.1016/j.atmosenv.2006.02.029.

Detection of viral aerosols by use of real-time quantitative PCR.

Wen Z, Yu L, Yang W, Wang J, Zhao J, Li N Aerobiologia (Bologna). 2020; 25(2):65-73.

PMID: 32214624 PMC: 7087933. DOI: 10.1007/s10453-009-9110-1.