Identification of in Vivo Expressed Vaccine Candidate Antigens from Staphylococcus Aureus

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2002 May 9

PMID 11997460

Citations 73

Authors

Hildegard Etz

Duc Bui Minh

Tamas Henics

Agnieszka Dryla

Birgit Winkler

Christine Triska

Aoife P Boyd

Johannes Sollner

Walter Schmidt

Uwe von Ahsen

Michael Buschle

Steven R Gill

James Kolonay

Hanif Khalak

Claire M Fraser

Alexander von Gabain

Eszter Nagy

Andreas Meinke

Affiliations

Soon will be listed here.

Abstract

For the design of potent subunit vaccines, it is of paramount importance to identify all antigens immunologically recognized by a patient population infected with a pathogen. We have developed a rapid and efficient procedure to identify such commonly recognized antigens, and here we provide a comprehensive in vivo antigenic profile of Staphylococcus aureus, an important human pathogen. S. aureus peptides were displayed on the surface of Escherichia coli via fusion to one of two outer membrane proteins (LamB and FhuA) and probed with sera selected for high Ab titer and opsonic activity. A total of 60 antigenic proteins were identified, most of which are located or predicted to be located on the surface of the bacterium or secreted. The identification of these antigens and their reactivity with individual sera from patients and healthy individuals greatly facilitate the selection of promising vaccine candidates for further evaluation. This approach, which makes use of whole genome sequence information, has the potential to greatly accelerate and facilitate the formulation of novel vaccines and is applicable to any pathogen that induces Abs in humans and/or experimental animals.

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