Human Monoclonal Natural Autoantibodies Against the T-cell Receptor Inhibit Interleukin-2 Production in Murine T Cells

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 2002 May 3

PMID 11985662

Citations 6

Authors

Ian F Robey

Samuel F Schluter

Emmanuel Akporiaye

David E Yocum

John J Marchalonis

Affiliations

Soon will be listed here.

Abstract

Natural autoantibodies (NAAbs) specific for the T-cell receptor (TCR) are present in all human sera, but individuals with rheumatoid arthritis (RA) generally produce higher titres of immunoglobulin M (IgM) isotype autoantibodies (AAbs) against Vbeta TCR epitopes. To investigate possible correlations between the specificity of such AAbs and their role in immunomodulation, we generated seven B-cell hetero-hybridomas, secreting monoclonal IgM NAAbs, from the synovial tissue and peripheral blood of patients with RA. Here we report three anti-TCR monoclonal autoantibodies (mAAbs)--OR2, OR5 and Syn 2H-11--with the ability to bind subsets of murine T cells, including the ovalbumin-specific DO-11.10 clone. These antibodies did not induce apoptosis in vitro, but prevented interleukin-2 (IL-2) production by antigen-specific T cells. These findings suggest an immunomodulatory function for NAAbs to TCR V-region epitopes and serve as the foundation for testing human anti-TCR mAAbs in animal models with the eventual goal of using them as therapeutic agents in human disease.

Citing Articles

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