The Membrane-associated Protein-serine/threonine Kinase from Sulfolobus Solfataricus is a Glycoprotein

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2002 Apr 27

PMID 11976289

Citations 15

Authors

Brian H Lower

Peter J Kennelly

Affiliations

Soon will be listed here.

Abstract

Treatment of a sodium dodecyl sulfate-polyacrylamide gel with periodic acid-Schiff (PAS) stain or blotting with Galanthus nivalis agglutinin revealed the presence of several glycosylated polypeptides in a partially purified detergent extract of the membrane fraction of Sulfolobus solfataricus. One of the glycoproteins comigrated with the membrane-associated protein-serine/threonine kinase from S. solfataricus, which had been radiolabeled by autophosphorylation with [(32)P]ATP in vitro. Treatment with a chemical deglycosylating agent, trifluoromethanesulfonic acid, abolished PAS staining and reduced the M(r) of the protein kinase from approximately 67,000 to approximately 62,000. Protein kinase activity also adhered to, and could be eluted from, agarose beads containing bound G. nivalis agglutinin. Glycosylation of the protein kinase implies that at least a portion of this integral membrane protein resides on the external surface of the cell membrane.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

SOLOW B, Bischoff K, Zylka M, Kennelly P . Archael phosphoproteins. Identification of a hexosephosphate mutase and the alpha-subunit of succinyl-CoA synthetase in the extreme acidothermophile Sulfolobus solfataricus. Protein Sci. 1998; 7(1):105-11. PMC: 2143807. DOI: 10.1002/pro.5560070111. View

Brock T, Brock K, Belly R, WEISS R . Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature. Arch Mikrobiol. 1972; 84(1):54-68. DOI: 10.1007/BF00408082. View

de Rosa M, Gambacorta A, Bulock J . Extremely thermophilic acidophilic bacteria convergent with Sulfolobus acidocaldarius. J Gen Microbiol. 1975; 86(1):156-64. DOI: 10.1099/00221287-86-1-156. View

Novogrodsky A, Lotan R, Ravid A, Sharon N . Peanut agglutinin, a new mitogen that binds to galactosyl sites exposed after neuraminidase treatment. J Immunol. 1975; 115(5):1243-8. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Spudich J, Stoeckenius W . Light-regulated retinal-dependent reversible phosphorylation of Halobacterium proteins. J Biol Chem. 1980; 255(12):5501-3. View

Wu J, Ohta N, Zhao J, Newton A . A novel bacterial tyrosine kinase essential for cell division and differentiation. Proc Natl Acad Sci U S A. 1999; 96(23):13068-73. PMC: 23901. DOI: 10.1073/pnas.96.23.13068. View

Lower B, Bischoff K, Kennelly P . The archaeon Sulfolobus solfataricus contains a membrane-associated protein kinase activity that preferentially phosphorylates threonine residues in vitro. J Bacteriol. 2000; 182(12):3452-9. PMC: 101924. DOI: 10.1128/JB.182.12.3452-3459.2000. View

10.

Eichler J . Novel glycoproteins of the halophilic archaeon Haloferax volcanii. Arch Microbiol. 2000; 173(5-6):445-8. DOI: 10.1007/s002030000152. View

11.

Koretke K, Lupas A, Warren P, Rosenberg M, Brown J . Evolution of two-component signal transduction. Mol Biol Evol. 2000; 17(12):1956-70. DOI: 10.1093/oxfordjournals.molbev.a026297. View

12.

Elferink M, Albers S, Konings W, Driessen A . Sugar transport in Sulfolobus solfataricus is mediated by two families of binding protein-dependent ABC transporters. Mol Microbiol. 2001; 39(6):1494-503. DOI: 10.1046/j.1365-2958.2001.02336.x. View

13.

Kim D, Forst S . Genomic analysis of the histidine kinase family in bacteria and archaea. Microbiology (Reading). 2001; 147(Pt 5):1197-1212. DOI: 10.1099/00221287-147-5-1197. View

14.

Schaffer C, Messner P . Glycobiology of surface layer proteins. Biochimie. 2001; 83(7):591-9. DOI: 10.1016/s0300-9084(01)01299-8. View

15.

Spudich E, Spudich J . Photosensitive phosphoproteins in Halobacteria: regulatory coupling of transmembrane proton flux and protein dephosphorylation. J Cell Biol. 1981; 91(3 Pt 1):895-900. PMC: 2112812. DOI: 10.1083/jcb.91.3.895. View

16.

Edge A, Faltynek C, Hof L, REICHERT Jr L, Weber P . Deglycosylation of glycoproteins by trifluoromethanesulfonic acid. Anal Biochem. 1981; 118(1):131-7. DOI: 10.1016/0003-2697(81)90168-8. View

17.

Skorko R . Protein phosphorylation in the archaebacterium Sulfolobus acidocaldarius. Eur J Biochem. 1984; 145(3):617-22. DOI: 10.1111/j.1432-1033.1984.tb08601.x. View

18.

Shibuya N, Goldstein I, Broekaert W, Nsimba-Lubaki M, Peeters B, Peumans W . The elderberry (Sambucus nigra L.) bark lectin recognizes the Neu5Ac(alpha 2-6)Gal/GalNAc sequence. J Biol Chem. 1987; 262(4):1596-601. View

19.

Yamashita K, Totani K, Ohkura T, Takasaki S, Goldstein I, Kobata A . Carbohydrate binding properties of complex-type oligosaccharides on immobilized Datura stramonium lectin. J Biol Chem. 1987; 262(4):1602-7. View

20.

Kennelly P, EDELMAN A, Blumenthal D, KREBS E . Rabbit skeletal muscle myosin light chain kinase. The calmodulin binding domain as a potential active site-directed inhibitory domain. J Biol Chem. 1987; 262(25):11958-63. View