Lipooligosaccharide and Polysaccharide Capsule: Virulence Factors of Neisseria Meningitidis That Determine Meningococcal Interaction with Human Dendritic Cells

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2002 Apr 16

PMID 11953382

Citations 33

Authors

Alexandra Unkmeir

Ulrike Kammerer

Anne Stade

Claudia Hubner

Sabine Haller

Annette Kolb-Maurer

Matthias Frosch

Guido Dietrich

Affiliations

Soon will be listed here.

Abstract

In this work we analyzed the roles of meningococcal lipooligosaccharide (LOS) and capsule expression in the interaction of Neisseria meningitidis with human dendritic cells (DC). Infection of DC with serogroup B wild-type meningococci induced a strong burst of the proinflammatory cytokines and chemokines tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-8. In contrast, a serogroup B mutant strain lacking LOS expression barely led to cytokine induction, demonstrating that meningococcal LOS is the main mediator of the proinflammatory response in human DC. Sialylation of meningococcal LOS did not influence cytokine secretion by DC. However, we found the phagocytosis of N. meningitidis by human DC to be inhibited by LOS sialylation. In addition, the expression of the meningococcal serogroup A, B, and C capsules dramatically reduced DC adherence of N. meningitidis and phagocytosis to some extent. Hence, LOS sialylation and capsule expression are independent mechanisms protecting N. meningitidis from the phagocytic activity of human DC.

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