Protection of Killer Antiidiotypic Antibodies Against Early Invasive Aspergillosis in a Murine Model of Allogeneic T-cell-depleted Bone Marrow Transplantation

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2002 Apr 16

PMID 11953373

Citations 24

Authors

Elio Cenci

Antonella Mencacci

Antonio Spreca

Claudia Montagnoli

Angela Bacci

Katia Perruccio

Andrea Velardi

Walter Magliani

Stefania Conti

Luciano Polonelli

Luigina Romani

Affiliations

Soon will be listed here.

Abstract

Antiidiotypic monoclonal antibodies (MAbs) representing the internal image of a yeast killer toxin (KT) have therapeutic potential against several fungal infections. The efficacy of KT MAbs against Aspergillus fumigatus was investigated in a mouse model of T-cell-depleted allogeneic bone marrow transplantation (BMT) with invasive pulmonary aspergillosis. Mice were highly susceptible to infection at 3 days post-BMT, when profound neutropenia was observed both in the periphery and in the lungs. Treatment with KT MAbs protected the mice from infection, as judged by the long-term survival and decreased pathology associated with inhibition of fungal growth and hyphal development in the lungs. In vitro, similar to polymorphonuclear neutrophils, KT MAbs significantly inhibited the hyphal development and metabolic activity of germinated Aspergillus conidia. These results indicate that mimicking the action of neutrophils could be a strategy through which KT MAbs exert therapeutic efficacy in A. fumigatus infections.

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