Bile Acids Induce Calcium Signals in Mouse Pancreatic Acinar Cells: Implications for Bile-induced Pancreatic Pathology

Overview

Journal J Physiol

Specialty Physiology

Date 2002 Apr 3

PMID 11927668

Citations 54

Authors

Svetlana Voronina

Rebecca Longbottom

Robert Sutton

Ole H Petersen

Alexei Tepikin

Affiliations

Soon will be listed here.

Abstract

The effect of the natural bile acid, taurolithocholic acid 3-sulfate (TLC-S), on calcium signalling in pancreatic acinar cells has been investigated. TLC-S induced global calcium oscillations and extended calcium transients as well as calcium signals localised to the secretory granule (apical) region of acinar cells. These calcium signals could still be triggered by TLC-S in a calcium-free external solution. TLC-S-induced calcium signals were not inhibited by atropine, but were abolished by caffeine or by depletion of calcium stores, due to prolonged application of ACh. Global calcium signals, produced by TLC-S application, displayed vectorial apical-to-basal polarity. The signals originated in the apical part and were then propagated to the basal region. Other natural bile acids, taurocholate (TC) and taurodeoxycholate (TDC), were also able to produce local and global calcium oscillations (but at higher concentrations than TLC-S). Bile, which can enter pancreas by reflux, has been implicated in the pathology of acute pancreatitis. The calcium releasing properties of bile acids suggest that calcium toxicity could be an important contributing factor in the bile acid-induced cellular damage.

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