The KLHL1-antisense Transcript ( KLHL1AS) is Evolutionarily Conserved

Overview

Journal Mamm Genome

Specialty Genetics

Date 2002 Mar 29

PMID 11919683

Citations 9

Authors

Kellie A Benzow

Michael D Koob

Affiliations

Soon will be listed here.

Abstract

Spinocerebellar ataxia type 8 (SCA8) is caused by a CTG expansion in an untranslated, endogenous antisense RNA that overlaps the Kelch-like 1 ( KLHL1) gene. The normal function of this transcript is currently unknown. We have now identified the promoter region for the KLHL1-antisense ( KLHL1AS) RNA and report that a Klhl1as transcript is present in the mouse as well. Human and mouse KLHL1AS are transcribed from homologous promoter regions in the first intron of KLHL1 and extend through the transcription and translation start sites as well as the first splice donor sequence of KLHL1. We found that the mouse Klhl1as RNA is not spliced and terminates in a polyadenylation site in the Klhl1 promoter region, whereas both the present and previous work show that human KLHL1AS is highly variably spliced into processed transcripts that contain up to six exons. Mouse Klhl1as transcript was detected in RNA isolated from the cerebellum and from total adult brain and total fetal tissue, and at a low level in testis and ovary. Similarly, human KLHL1AS is expressed in various brain tissues, including the cerebellum, the tissue most affected by SCA8, and was detected at low levels in testis and kidney. The evolutionary conservation of this antisense/sense transcriptional organization strongly indicates that KLHL1AS transcripts play a significant biological role in both human and mouse, presumably as a regulator of KLHL1 expression.

Citing Articles

Antisense Transcription across Nucleotide Repeat Expansions in Neurodegenerative and Neuromuscular Diseases: Progress and Mysteries.

Castro A, Loureiro J, Bessa J, Silveira I Genes (Basel). 2020; 11(12).

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Internal ribosome entry segment activity of ATXN8 opposite strand RNA.

Chen I, Lin H, Hsiao Y, Chen C, Wu Y, Shiau H PLoS One. 2013; 8(9):e73885.

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Bidirectional expression of the SCA8 expansion mutation: one mutation, two genes.

Ikeda Y, Daughters R, Ranum L Cerebellum. 2008; 7(2):150-8.

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Targeted deletion of a single Sca8 ataxia locus allele in mice causes abnormal gait, progressive loss of motor coordination, and Purkinje cell dendritic deficits.

He Y, Zu T, Benzow K, Orr H, Clark H, Koob M J Neurosci. 2006; 26(39):9975-82.

PMID: 17005861 PMC: 6674467. DOI: 10.1523/JNEUROSCI.2595-06.2006.

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