Alpha 2.0
Login Register
» Articles » PMID: 11916735

Effects of PH and Magnetic Material on Immunomagnetic Separation of Cryptosporidium Oocysts from Concentrated Water Samples

Overview
Journal Appl Environ Microbiol
Specialties Environmental Health
Microbiology
Date 2002 Mar 28
PMID 11916735
Citations 9
Authors
Ryan C Kuhn
Channah M Rock
Kevin H Oshima
Affiliations
Soon will be listed here.
Abstract

In this study, we examined the effect that magnetic materials and pH have on the recoveries of Cryptosporidium oocysts by immunomagnetic separation (IMS). We determined that particles that were concentrated on a magnet during bead separation have no influence on oocyst recovery; however, removal of these particles did influence pH values. The optimal pH of the IMS was determined to be 7.0. The numbers of oocysts recovered from deionized water at pH 7.0 were 26.3% higher than those recovered from samples that were not at optimal pH. The results indicate that the buffers in the IMS kit did not adequately maintain an optimum pH in some water samples. By adjusting the pH of concentrated environmental water samples to 7.0, recoveries of oocysts increased by 26.4% compared to recoveries from samples where the pH was not adjusted.

Citing Articles

An automated bacterial concentration and recovery system for pre-enrichment required in rapid Escherichia coli detection.

Zhang Y, Xu C, Guo T, Hong L Sci Rep. 2018; 8(1):17808.

PMID: 30546076 PMC: 6292886. DOI: 10.1038/s41598-018-35970-8.

Evaluation of an Ultrafiltration-Based Procedure for Simultaneous Recovery of Diverse Microbes in Source Waters.

Kahler A, Johnson T, Hahn D, Narayanan J, Derado G, Hill V Water (Basel). 2015; 7(3):1202-1216.

PMID: 26530003 PMC: 4627901. DOI: 10.3390/w7031202.

Methods to detect infectious human enteric viruses in environmental water samples.

Hamza I, Jurzik L, Uberla K, Wilhelm M Int J Hyg Environ Health. 2011; 214(6):424-36.

PMID: 21920815 PMC: 7106513. DOI: 10.1016/j.ijheh.2011.07.014.

Cryptosporidium oocyst detection in water samples: floatation technique enhanced with immunofluorescence is as effective as immunomagnetic separation method.

Koompapong K, Sutthikornchai C, Sukthana Y Korean J Parasitol. 2009; 47(4):353-7.

PMID: 19967082 PMC: 2788713. DOI: 10.3347/kjp.2009.47.4.353.

Improved risk analysis by dual direct detection of total and infectious Cryptosporidium oocysts on cell culture in combination with immunofluorescence assay.

Lalancette C, Di Giovanni G, Prevost M Appl Environ Microbiol. 2009; 76(2):566-77.

PMID: 19933339 PMC: 2805227. DOI: 10.1128/AEM.01496-09.

References
1.
Simmons 3rd O, Sobsey M, Heaney C, Schaefer 3rd F, Francy D . Concentration and detection of cryptosporidium oocysts in surface water samples by method 1622 using ultrafiltration and capsule filtration. Appl Environ Microbiol. 2001; 67(3):1123-7. PMC: 92704. DOI: 10.1128/AEM.67.3.1123-1127.2001. View
2.
Kuhn R, Oshima K . Evaluation and optimization of a reusable hollow fiber ultrafilter as a first step in concentrating Cryptosporidium parvum oocysts from water. Water Res. 2001; 35(11):2779-83. DOI: 10.1016/s0043-1354(00)00560-1. View
3.
Rochelle P, de Leon R, Johnson A, Stewart M, Wolfe R . Evaluation of immunomagnetic separation for recovery of infectious Cryptosporidium parvum oocysts from environmental samples. Appl Environ Microbiol. 1999; 65(2):841-5. PMC: 91105. DOI: 10.1128/AEM.65.2.841-845.1999. View
4.
Bukhari Z, McCuin R, Fricker C, Clancy J . Immunomagnetic separation of Cryptosporidium parvum from source water samples of various turbidities. Appl Environ Microbiol. 1998; 64(11):4495-9. PMC: 106675. DOI: 10.1128/AEM.64.11.4495-4499.1998. View
5.
Kilani R, Sekla L . Purification of Cryptosporidium oocysts and sporozoites by cesium chloride and Percoll gradients. Am J Trop Med Hyg. 1987; 36(3):505-8. DOI: 10.4269/ajtmh.1987.36.505. View