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Growth-associated Production of Poly-3-hydroxybutyrate by Bacillus Mycoides

Overview
Journal Folia Microbiol (Praha)
Publisher Springer
Specialty Microbiology
Date 2002 Mar 20
PMID 11898337
Citations 3
Authors
P S Thakur
B Borah
S D Baruah
J N Nigam
Affiliations
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Abstract

Bacillus mycoides strain RIJ B-017, a growth-associated poly-3-hydroxybutyrate (PHB) producer was grown on sucrose-containing media. PHB accumulated in cells up to 72% of dry cell mass. The overall maximum value of PHB yield (Yp/s) and productivities (Qp and qp) 250 mgp/gs, 120 mgp L-1 h-1 and 30 mgp gx-1 h-1, respectively, were obtained at 15 g/L sucrose. Differential scanning calorimeter heating curve showed two peaks, one at 95.9 degrees C and another at 165.4 degrees C with a shoulder around 154.6 degrees C. The viscosity-average molar mass in chloroform at 27 degrees C was 505 kDa. The carbon content of PHB was 55.4% of the mass.

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