Late Transient Expression of Human Hepatitis B Virus Genes in Monkey Cells

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1983 Jan 1

PMID 11894903

Citations 6

Authors

F Colbere-Garapin

F HORODNICEANU

P Kourilsky

A C Garapin

Affiliations

Soon will be listed here.

Abstract

The expression of human hepatitis B virus (HBV) surface (HBS) and e (HBe) antigens has been studied comparatively in monkey and mouse cell lines co-transfected with HBV DNA and the dominant selective marker aminoglycoside 3'-phosphotransferase gene. We have found that the kinetics and stability of expression of the HBS gene varies with the cell lines used. Only a late transient expression of both HBS and HBe is observed between 1 and 5 weeks after transfection in monkey kidney Vero cells transfected with the complete HBV genome, while a permanent expression of HBS and HBe is obtained in mouse cells. HBS and HBe are excreted into the cell culture medium. HBe is expressed in cells transfected with the complete HBV genome, but not with isolated HBS gene. In clones of Vero cells transformed with the HBS gene, HBV sequences were rearranged or lost.

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References

Colbere-Garapin F, HORODNICEANU F, Kourilsky P, Garapin A . A new dominant hybrid selective marker for higher eukaryotic cells. J Mol Biol. 1981; 150(1):1-14. DOI: 10.1016/0022-2836(81)90321-1. View

Moriarty A, HOYER B, Shih J, Gerin J, Hamer D . Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector. Proc Natl Acad Sci U S A. 1981; 78(4):2606-10. PMC: 319398. DOI: 10.1073/pnas.78.4.2606. View

Ogston C, Jonak G, Rogler C, Astrin S, Summers J . Cloning and structural analysis of integrated woodchuck hepatitis virus sequences from hepatocellular carcinomas of woodchucks. Cell. 1982; 29(2):385-94. DOI: 10.1016/0092-8674(82)90155-6. View

Pourcel C, Louise A, Gervais M, Chenciner N, Dubois M, Tiollais P . Transcription of the hepatitis B surface antigen gene in mouse cells transformed with cloned viral DNA. J Virol. 1982; 42(1):100-5. PMC: 256049. DOI: 10.1128/JVI.42.1.100-105.1982. View

Christman J, Gerber M, Price P, Flordellis C, Edelman J, Acs G . Amplification of expression of hepatitis B surface antigen in 3T3 cells cotransfected with a dominant-acting gene and cloned viral DNA. Proc Natl Acad Sci U S A. 1982; 79(6):1815-9. PMC: 346071. DOI: 10.1073/pnas.79.6.1815. View

Summers J, Mason W . Replication of the genome of a hepatitis B--like virus by reverse transcription of an RNA intermediate. Cell. 1982; 29(2):403-15. DOI: 10.1016/0092-8674(82)90157-x. View

Graham F, Van Der Eb A . A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973; 52(2):456-67. DOI: 10.1016/0042-6822(73)90341-3. View

Petricciani J, Kirchstein R, Hines J, WALLACE R, Martin D . Tumorigenicity assays in nonhuman primates treated with antithymocyte globulin. J Natl Cancer Inst. 1973; 51(1):191-6. DOI: 10.1093/jnci/51.1.191. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

10.

Alter H, Seeff L, KAPLAN P, McAuliffe V, Wright E, Gerin J . Type B hepatitis: the infectivity of blood positive for e antigen and DNA polymerase after accidental needlestick exposure. N Engl J Med. 1976; 295(17):909-13. DOI: 10.1056/NEJM197610212951701. View

11.

Alexander J, Bey E, Geddes E, Lecatsas G . Establishment of a continuously growing cell line from primary carcinoma of the liver. S Afr Med J. 1976; 50(54):2124-8. View

12.

Murray N, Brammar W, Murray K . Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet. 1977; 150(1):53-61. DOI: 10.1007/BF02425325. View

13.

Davidson R, GERALD P . Induction of mammalian somatic cell hybridization by polyethylene glycol. Methods Cell Biol. 1977; 15:325-38. DOI: 10.1016/s0091-679x(08)60223-x. View

14.

Garapin A, LePennec J, Roskam W, Perrin F, Cami B, Krust A . Isolation by molecular cloning of a fragment in the split ovalbumin gene. Nature. 1978; 273(5661):349-54. DOI: 10.1038/273349a0. View

15.

Takahashi K, Akahane Y, Gotanda T, Mishiro T, Imai M, Miyakawa Y . Demonstration of hepatitis B e antigen in the core of Dane particles. J Immunol. 1979; 122(1):275-9. View

16.

Charnay P, Pourcel C, Louise A, Fritsch A, Tiollais P . Cloning in Escherichia coli and physical structure of hepatitis B virion DNA. Proc Natl Acad Sci U S A. 1979; 76(5):2222-6. PMC: 383570. DOI: 10.1073/pnas.76.5.2222. View

17.

Colbere-Garapin F, Chousterman S, HORODNICEANU F, Kourilsky P, Garapin A . Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1979; 76(8):3755-9. PMC: 383912. DOI: 10.1073/pnas.76.8.3755. View

18.

Rothstein S, Jorgensen R, Postle K, Reznikoff W . The inverted repeats of Tn5 are functionally different. Cell. 1980; 19(3):795-805. DOI: 10.1016/s0092-8674(80)80055-9. View

19.

Galibert F, Mandart E, Fitoussi F, Tiollais P, Charnay P . Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature. 1979; 281(5733):646-50. DOI: 10.1038/281646a0. View

20.

Gluzman Y, Frisque R, Sambrook J . Origin-defective mutants of SV40. Cold Spring Harb Symp Quant Biol. 1980; 44 Pt 1,:293-300. DOI: 10.1101/sqb.1980.044.01.033. View