Metabolic Signals That Lead to Control of CBB Gene Expression in Rhodobacter Capsulatus

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2002 Mar 13

PMID 11889097

Citations 12

Authors

Mary A Tichi

F Robert Tabita

Affiliations

Soon will be listed here.

Abstract

Various mutant strains were used to examine the regulation and metabolic control of the Calvin-Benson-Bassham (CBB) reductive pentose phosphate pathway in Rhodobacter capsulatus. Previously, a ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO)-deficient strain (strain SBI/II) was found to show enhanced levels of cbb(I) and cbb(II) promoter activities during photoheterotrophic growth in the presence of dimethyl sulfoxide. With this strain as the starting point, additional mutations were made in genes encoding phosphoribulokinase and transketolase and in the gene encoding the LysR-type transcriptional activator, CbbR(II). These strains revealed that a product generated by phosphoribulokinase was involved in control of CbbR-mediated cbb gene expression in SBI/II. Additionally, heterologous expression experiments indicated that Rhodobacter sphaeroides CbbR responded to the same metabolic signal in R. capsulatus SBI/II and mutant strain backgrounds.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Grimberg J, Maguire S, Belluscio L . A simple method for the preparation of plasmid and chromosomal E. coli DNA. Nucleic Acids Res. 1989; 17(21):8893. PMC: 335085. DOI: 10.1093/nar/17.21.8893. View

Tabita F . Pyridine nucleotide control and subunit structure of phosphoribulokinase from photosynthetic bacteria. J Bacteriol. 1980; 143(3):1275-80. PMC: 294495. DOI: 10.1128/jb.143.3.1275-1280.1980. View

Dijkhuizen L, Harder W . Current views on the regulation of autotrophic carbon dioxide fixation via the Calvin cycle in bacteria. Antonie Van Leeuwenhoek. 1984; 50(5-6):473-87. DOI: 10.1007/BF02386221. View

Chen J, Gibson J, McCue L, Tabita F . Identification, expression, and deduced primary structure of transketolase and other enzymes encoded within the form II CO2 fixation operon of Rhodobacter sphaeroides. J Biol Chem. 1991; 266(30):20447-52. View

Bauer C, Bird T . Regulatory circuits controlling photosynthesis gene expression. Cell. 1996; 85(1):5-8. DOI: 10.1016/s0092-8674(00)81074-0. View

Penfold R, Pemberton J . An improved suicide vector for construction of chromosomal insertion mutations in bacteria. Gene. 1992; 118(1):145-6. DOI: 10.1016/0378-1119(92)90263-o. View

Duport C, Meyer C, Naud I, Jouanneau Y . A new gene expression system based on a fructose-dependent promoter from Rhodobacter capsulatus. Gene. 1994; 145(1):103-8. DOI: 10.1016/0378-1119(94)90330-1. View

Shively J, Davidson E, Marrs B . Depression of the synthesis of the intermediate and large forms of ribulose-1,5-bisphosphate carboxylase/oxygenase in Rhodopseudomonas capsulata. Arch Microbiol. 1984; 138(3):233-6. DOI: 10.1007/BF00402127. View

10.

van Keulen G, Girbal L, van den Bergh E, Dijkhuizen L, Meijer W . The LysR-type transcriptional regulator CbbR controlling autotrophic CO2 fixation by Xanthobacter flavus is an NADPH sensor. J Bacteriol. 1998; 180(6):1411-7. PMC: 107038. DOI: 10.1128/JB.180.6.1411-1417.1998. View

11.

Mosley C, Suzuki J, Bauer C . Identification and molecular genetic characterization of a sensor kinase responsible for coordinately regulating light harvesting and reaction center gene expression in response to anaerobiosis. J Bacteriol. 1994; 176(24):7566-73. PMC: 197214. DOI: 10.1128/jb.176.24.7566-7573.1994. View

12.

Gibson J, Tabita F . Nucleotide sequence and functional analysis of cbbR, a positive regulator of the Calvin cycle operons of Rhodobacter sphaeroides. J Bacteriol. 1993; 175(18):5778-84. PMC: 206655. DOI: 10.1128/jb.175.18.5778-5784.1993. View

13.

Paoli G, Soyer F, Shively J, Tabita F . Rhodobacter capsulatus genes encoding form I ribulose-1,5-bisphosphate carboxylase/oxygenase (cbbLS) and neighbouring genes were acquired by a horizontal gene transfer. Microbiology (Reading). 1998; 144 ( Pt 1):219-227. DOI: 10.1099/00221287-144-1-219. View

14.

Falcone D, Tabita F . Expression of endogenous and foreign ribulose 1,5-bisphosphate carboxylase-oxygenase (RubisCO) genes in a RubisCO deletion mutant of Rhodobacter sphaeroides. J Bacteriol. 1991; 173(6):2099-108. PMC: 207746. DOI: 10.1128/jb.173.6.2099-2108.1991. View

15.

de Sury dAspremont R, Toussaint B, Vignais P . Isolation of Rhodobacter capsulatus transketolase: cloning and sequencing of its structural tktA gene. Gene. 1996; 169(1):81-4. DOI: 10.1016/0378-1119(95)00796-2. View

16.

Eraso J, Kaplan S . prrA, a putative response regulator involved in oxygen regulation of photosynthesis gene expression in Rhodobacter sphaeroides. J Bacteriol. 1994; 176(1):32-43. PMC: 205011. DOI: 10.1128/jb.176.1.32-43.1994. View

17.

Wang X, Falcone D, Tabita F . Reductive pentose phosphate-independent CO2 fixation in Rhodobacter sphaeroides and evidence that ribulose bisphosphate carboxylase/oxygenase activity serves to maintain the redox balance of the cell. J Bacteriol. 1993; 175(11):3372-9. PMC: 204734. DOI: 10.1128/jb.175.11.3372-3379.1993. View

18.

Schell M . Molecular biology of the LysR family of transcriptional regulators. Annu Rev Microbiol. 1993; 47:597-626. DOI: 10.1146/annurev.mi.47.100193.003121. View

19.

Ormerod J, ORMEROD K, Gest H . Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. Arch Biochem Biophys. 1961; 94:449-63. DOI: 10.1016/0003-9861(61)90073-x. View

20.

Hallenbeck P, Lerchen R, Hessler P, Kaplan S . Roles of CfxA, CfxB, and external electron acceptors in regulation of ribulose 1,5-bisphosphate carboxylase/oxygenase expression in Rhodobacter sphaeroides. J Bacteriol. 1990; 172(4):1736-48. PMC: 208664. DOI: 10.1128/jb.172.4.1736-1748.1990. View