Conglutinin Binding Polyethylene Glycol Precipitation Assay for Immune Complexes

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1979 Nov 1

PMID 118838

Citations 18

Authors

M Macanovic

P J LACHMANN

Affiliations

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Abstract

An assay for circulating immune complexes is described which uses radiolabelled bovine conglutinin as ligand and polyethylene glycol precipitation as the technique for separating bound ligands. The technique is simple to perform and gives good sensitivity detecting artificial immune complexes. Its use in detecting complexes in systemic lupus erythematosus and Burkitt's lymphoma is described and it is compared with the Clq binding assay also performed with polyethylene glycol. It is suggested that the simultaneous performance of polyethylene glycol assays using radiolabelled Clq and radiolabelled conglutinin may be an advantageous method for screening sera for the presence of immune complexes.

Citing Articles

The paraproteins in systemic capillary leak syndrome.

Zhang W, Ewan P, LACHMANN P Clin Exp Immunol. 1993; 93(3):424-9.

PMID: 8370170 PMC: 1554918. DOI: 10.1111/j.1365-2249.1993.tb08195.x.

Radioconglutinin-binding azssay for circulating immune complexes: a new method.

Johny K, Dasgupta M, Singh B, Dossetor J Clin Exp Immunol. 1980; 40(3):459-68.

PMID: 7418262 PMC: 1538933.

The use of C1q, conglutinin and low affinity rabbit IgM antibody to human Fc in a ligand coctail radioassay for detecting and characterizing immune complexes in pathological sera.

Harkiss G, Brown D Clin Exp Immunol. 1980; 39(3):576-82.

PMID: 7379330 PMC: 1538118.

Isolation of circulating immune complexes by conglutinin and separation of antigen from dissociated complexes by immobilized protein A.

Gupta R, Tan E Clin Exp Immunol. 1981; 46(1):9-19.

PMID: 7337970 PMC: 1536347.

Evaluation of six tests for circulating IgG complexes with special reference to IgM rheumatoid factors: analysis of systemic lupus erythematosus and rheumatoid arthritis series.

Wager O, Lindstrom P, Rasanen J, Kekomaki R, Ziola B, Salmi A Clin Exp Immunol. 1981; 46(1):149-60.

PMID: 7337967 PMC: 1536341.

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