Crystal Structures of 7-methylguanosine 5'-triphosphate (m(7)GTP)- and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)-bound Human Full-length Eukaryotic Initiation Factor 4E: Biological Importance of the C-terminal Flexible...

Overview

Journal Biochem J

Specialty Biochemistry

Date 2002 Mar 7

PMID 11879179

Citations 57

Authors

Koji Tomoo

Xu Shen

Koumei Okabe

Yoshiaki Nozoe

Shoichi Fukuhara

Shigenobu Morino

Toshimasa Ishida

Taizo Taniguchi

Hiroshi Hasegawa

Akira Terashima

Masahiro Sasaki

Yoshio Katsuya

Kunihiro Kitamura

Hiroshi Miyoshi

Masahide Ishikawa

Kin-ichiro Miura

Affiliations

Soon will be listed here.

Abstract

The crystal structures of the full-length human eukaryotic initiation factor (eIF) 4E complexed with two mRNA cap analogues [7-methylguanosine 5'-triphosphate (m(7)GTP) and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)] were determined at 2.0 A resolution (where 1 A=0.1 nm). The flexibility of the C-terminal loop region of eIF4E complexed with m(7)GTP was significantly reduced when complexed with m(7)GpppA, suggesting the importance of the second nucleotide in the mRNA cap structure for the biological function of eIF4E, especially the fixation and orientation of the C-terminal loop region, including the eIF4E phosphorylation residue. The present results provide the structural basis for the biological function of both N- and C-terminal mobile regions of eIF4E in translation initiation, especially the regulatory function through the switch-on/off of eIF4E-binding protein-eIF4E phosphorylation.

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