Improved Outcome of Chronic Pseudomonas Aeruginosa Lung Infection is Associated with Induction of a Th1-dominated Cytokine Response

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 2002 Mar 6

PMID 11876741

Citations 43

Authors

C Moser

P O Jensen

O Kobayashi

H P Hougen

Z Song

J Rygaard

A Kharazmi

N H by

Affiliations

Soon will be listed here.

Abstract

Repeated challenge with antigen is involved in the pathogenesis of a variety of pulmonary diseases. Patients with cystic fibrosis (CF) experience recurrent pulmonary colonization with Pseudomonas aeruginosa before establishment of chronic lung infection. To mimic recurrent lung infections in CF patients, the lungs of susceptible BALB/c mice were re-infected with P. aeruginosa 14 days after the initial infection. Singly-infected BALB/c mice, as well as non-infected mice, were used as controls. Decreased mortality and milder lung inflammation in re-infected BALB/c mice, as well as a tendency for improved clearance of bacteria, was observed when compared with singly-infected mice. The improved outcome in re-infected mice correlated with changes in CD4 cell numbers. Surface expression of LFA-1 on pulmonary CD4 cells was increased in re-infected compared with singly-infected mice. Moreover, resistance to re-infection was paralleled by a shift towards a Th1-dominated response and increased IL-12 production. No significant increase in serum IgG was observed in the re-infected mice. In conclusion, these results indicate a protective role for a Th1-dominated response, independent of antibody production, in chronic P. aeruginosa lung infection in CF.

Citing Articles

in chronic lung disease: untangling the dysregulated host immune response.

Nickerson R, Thornton C, Johnston B, Lee A, Cheng Z Front Immunol. 2024; 15:1405376.

PMID: 39015565 PMC: 11250099. DOI: 10.3389/fimmu.2024.1405376.

Strong immune responses and protection of PcrV and OprF-I mRNA vaccine candidates against Pseudomonas aeruginosa.

Wang X, Liu C, Rcheulishvili N, Papukashvili D, Xie F, Zhao J NPJ Vaccines. 2023; 8(1):76.

PMID: 37231060 PMC: 10209580. DOI: 10.1038/s41541-023-00672-4.

Synthesis and Evaluation of a Self-Adjuvanting Pseudomonal Vaccine Based on Major Outer Membrane Porin OprF Epitopes Formulated with Low-Toxicity QS-21-Containing Liposomes.

Thanvi R, Nada S, Dissanayake R, Vartak A, Sebilleau C, Alom N Bioconjug Chem. 2023; 34(5):893-910.

PMID: 37092892 PMC: 10723056. DOI: 10.1021/acs.bioconjchem.3c00103.

Overcoming Barriers to Preventing and Treating Infections Using AAV Vectored Immunoprophylaxis.

Lopes J, Rghei A, Thompson B, Susta L, Khursigara C, Wootton S Biomedicines. 2022; 10(12).

PMID: 36551918 PMC: 9775905. DOI: 10.3390/biomedicines10123162.

Modulation of immune cell function, IDO expression and kynurenine production by the quorum sensor 2-heptyl-3-hydroxy-4-quinolone (PQS).

Ogbechi J, Huang Y, Clanchy F, Pantazi E, Topping L, Darlington L Front Immunol. 2022; 13:1001956.

PMID: 36389710 PMC: 9650388. DOI: 10.3389/fimmu.2022.1001956.

References

Markham R, Powderly W . Exposure of mice to live Pseudomonas aeruginosa generates protective cell-mediated immunity in the absence of an antibody response. J Immunol. 1988; 140(6):2039-45. View

Mathee K, Ciofu O, Sternberg C, Lindum P, Campbell J, Jensen P . Mucoid conversion of Pseudomonas aeruginosa by hydrogen peroxide: a mechanism for virulence activation in the cystic fibrosis lung. Microbiology (Reading). 1999; 145 ( Pt 6):1349-1357. DOI: 10.1099/13500872-145-6-1349. View

Salomon B, Bluestone J . LFA-1 interaction with ICAM-1 and ICAM-2 regulates Th2 cytokine production. J Immunol. 1998; 161(10):5138-42. View

Wojnarowski C, Frischer T, Hofbauer E, Grabner C, Mosgoeller W, Eichler I . Cytokine expression in bronchial biopsies of cystic fibrosis patients with and without acute exacerbation. Eur Respir J. 1999; 14(5):1136-44. DOI: 10.1183/09031936.99.14511369. View

Moser C, Hougen H, Song Z, Rygaard J, Kharazmi A, Hoiby N . Early immune response in susceptible and resistant mice strains with chronic Pseudomonas aeruginosa lung infection determines the type of T-helper cell response. APMIS. 2000; 107(12):1093-100. DOI: 10.1111/j.1699-0463.1999.tb01514.x. View

DAmbrosio D, Iellem A, Colantonio L, Clissi B, Pardi R, Sinigaglia F . Localization of Th-cell subsets in inflammation: differential thresholds for extravasation of Th1 and Th2 cells. Immunol Today. 2000; 21(4):183-6. DOI: 10.1016/s0167-5699(00)01590-5. View

Todt J, Sonstein J, Polak T, Seitzman G, Hu B, Curtis J . Repeated intratracheal challenge with particulate antigen modulates murine lung cytokines. J Immunol. 2001; 164(8):4037-47. DOI: 10.4049/jimmunol.164.8.4037. View

Moser C, Kjaergaard S, Pressler T, Kharazmi A, Koch C, Hoiby N . The immune response to chronic Pseudomonas aeruginosa lung infection in cystic fibrosis patients is predominantly of the Th2 type. APMIS. 2000; 108(5):329-35. DOI: 10.1034/j.1600-0463.2000.d01-64.x. View

Filip C, Fletcher G, Wulff J, Earhart C . Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol. 1973; 115(3):717-22. PMC: 246312. DOI: 10.1128/jb.115.3.717-722.1973. View

10.

Hoiby N, FLENSBORG E, Beck B, Friis B, Jacobsen S, Jacobsen L . Pseudomonas aeruginosa infection in cystic fibrosis. Diagnostic and prognostic significance of Pseudomonas aeruginosa precipitins determined by means of crossed immunoelectrophoresis. Scand J Respir Dis. 1977; 58(2):65-79. View

11.

Sorensen R, Stern R, POLMAR S . Cellular immunity to bacteria: impairment of in vitro lymphocyte responses to Pseudomonas aeruginosa in cystic fibrosis patients. Infect Immun. 1977; 18(3):735-40. PMC: 421296. DOI: 10.1128/iai.18.3.735-740.1977. View

12.

BEHIN R, Mauel J, Sordat B . Leishmania tropica: pathogenicity and in vitro macrophage function in strains of inbred mice. Exp Parasitol. 1979; 48(1):81-91. DOI: 10.1016/0014-4894(79)90057-2. View

13.

Shand G, Anwar H, Kadurugamuwa J, Brown M, Silverman S, Melling J . In vivo evidence that bacteria in urinary tract infection grow under iron-restricted conditions. Infect Immun. 1985; 48(1):35-9. PMC: 261910. DOI: 10.1128/iai.48.1.35-39.1985. View

14.

Mosmann T, Cherwinski H, Bond M, Giedlin M, Coffman R . Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986; 136(7):2348-57. View

15.

Powderly W, Pier G, Markham R . T lymphocyte-mediated protection against Pseudomonas aeruginosa infection in granulocytopenic mice. J Clin Invest. 1986; 78(2):375-80. PMC: 423557. DOI: 10.1172/JCI112587. View

16.

Markham R, Pier G, Powderly W . Suppressor T cells regulating the cell-mediated immune response to Pseudomonas aeruginosa can be generated by immunization with anti-bacterial T cells. J Immunol. 1988; 141(11):3975-9. View

17.

Belosevic M, Finbloom D, van der Meide P, Slayter M, Nacy C . Administration of monoclonal anti-IFN-gamma antibodies in vivo abrogates natural resistance of C3H/HeN mice to infection with Leishmania major. J Immunol. 1989; 143(1):266-74. View

18.

Baltimore R, Christie C, Smith G . Immunohistopathologic localization of Pseudomonas aeruginosa in lungs from patients with cystic fibrosis. Implications for the pathogenesis of progressive lung deterioration. Am Rev Respir Dis. 1989; 140(6):1650-61. DOI: 10.1164/ajrccm/140.6.1650. View

19.

Pedersen S, Shand G, Hansen B, HANSEN G . Induction of experimental chronic Pseudomonas aeruginosa lung infection with P. aeruginosa entrapped in alginate microspheres. APMIS. 1990; 98(3):203-11. View

20.

Yamamura M, Uyemura K, Deans R, Weinberg K, Rea T, Bloom B . Defining protective responses to pathogens: cytokine profiles in leprosy lesions. Science. 1991; 254(5029):277-9. DOI: 10.1126/science.254.5029.277. View